Evidence map›Paper›PMID 37277792›Full record

ArticleHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology2023

Effects of Foxp3 gene silencing on the expression of inflammatory cytokines and the proliferation and migration of human periodontal ligament fibroblasts in an inflammatory environment.

Ting Lu, Jiahao Zhu, Shihe Yang, Zhe Shen, Liangjun Zhong

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ting LuSchool of Stomatology, Hangzhou Normal University, Hangzhou 310000, China.
Jiahao ZhuSchool of Stomatology, Hangzhou Normal University, Hangzhou 310000, China.
Shihe YangSchool of Stomatology, Hangzhou Normal University, Hangzhou 310000, China.
Zhe ShenSchool of Stomatology, Hangzhou Normal University, Hangzhou 310000, China.
Liangjun ZhongSchool of Stomatology, Hangzhou Normal University, Hangzhou 310000, China.
Hangzhou Normal University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study aimed to clarify the effects of Foxp3 silencing on the expression of inflammatory cytokines in human periodontal ligament cells (hPDLFs) in an inflammatory environment and on cell proliferation and invasiveness, as well as to explore the role of Foxp3 gene in the development of periodontitis.

methodsAn small interfering RNA (siRNA) construct specific for Foxp3 was transfected into hPDLFs. Foxp3 silencing efficiency was verified by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting, and the siRNA with the optimum silencing effect of Foxp3 gene was screened. Using lipopolysaccharide to simulate an inflammatory environment

resultsAfter siRNA transfection, RT-PCR and Western blotting analyses showed that the expression of Foxp3 mRNA in the Foxp3-si3 group decreased significantly (

conclusionsIn an inflammatory environment, Foxp3 gene silencing promoted hPDLFs migration but had no significant effect on hPDLFs proliferation. The expression of inflammatory factors expressed in hPDLFs increased after Foxp3 gene silencing, indicating that Foxp3 gene inhibited inflammation in periodontitis.

Indexed as

CytokinesPeriodontitisCell ProliferationCells, CulturedFibroblastsForkhead Transcription FactorsGene SilencingHumansInterleukin-6Interleukin-8Periodontal LigamentRNA, Small InterferingTranscription FactorsCytokinesForkhead Transcription FactorsFOXP3 protein, humanInterleukin-6Interleukin-8RNA, Small InterferingTranscription FactorsFoxp3human periodontal ligament fibroblastslipopolysaccharideperio-dontitissmall interfering RNA

Identifiers

PMID37277792
PMCPMC10317850
OpenAlexW4379508635

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.