Evidence map›Paper›PMID 37283933›Full record

ArticleFree neuropathology2023

Golgi localization of SARS-CoV-2 spike protein and interaction with furin in cerebral COVID-19 microangiopathy: a clue to the central nervous system involvement?

Susana Boluda, Karima Mokhtari, Bruno Mégarbane, Djillali Annane, Bertrand Mathon, Albert Cao, Clovis Adam, Alexandre Androuin, Franck Bielle, Guy Brochier and 25 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Free neuropathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors at 6 institutions in 1 country.

Susana BoludaDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Karima MokhtariDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Bruno MégarbaneDepartment of Medical and Toxicological Critical Care, Lariboisière Hospital, AP-HP, Paris University, INSERM UMRS-1144, Paris, France.
Djillali AnnaneDepartment of Critical Care, Raymond Poincaré Hospital, Boulevard Raymond Poincaré, APHP, Paris-Saclay University, INSERM U1173, Garches, France.
Bertrand MathonDepartment of Neurosurgery, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Albert CaoDepartment of Neurology, Neuro-ICU, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Clovis AdamDepartment of Pathology, Bicêtre Hospital, AP-HP, Paris Saclay University, Le Kremlin-Bicêtre, France.
Alexandre AndrouinInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Franck BielleDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Guy BrochierDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Frédéric CharlotteDepartment of Pathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Lydia ChougarDepartment of Neuroimaging, Pitié-Salpètrière Hospital, AP-HP Sorbonne University, Paris, France.
Khalid Hamid El HachimiInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Marc EloitInstitut Pasteur, Pathogen Discovery Laboratory, Paris, France.
Stéphane HaïkDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Dominique HervéDepartment of Neurology, Lariboisière Hospital, AP-HP Nord- Paris University, Paris, France.
Amal KasriInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Valentin LeducqDepartment of Virology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, INSERM 1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Paris, France.
Stéphane LehéricyDepartment of Neuroimaging, Pitié-Salpètrière Hospital, AP-HP Sorbonne University, Paris, France.
Etienne LevavasseurDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Christian LobsigerInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Geoffroy Lorin de La GrandmaisonDepartment of Forensic Medicine and Pathology, Raymond Poincaré Hospital, AP-HP, Paris Saclay University, Garches, France.
Isabelle MaletDepartment of Virology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, INSERM 1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Paris, France.
Isabelle MalissinDepartment of Medical and Toxicological Critical Care, Lariboisière Hospital, AP-HP, Paris University, INSERM UMRS-1144, Paris, France.
Stéphane MarotDepartment of Virology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, INSERM 1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Paris, France.
Serge MartyInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Philippe PérotInstitut Pasteur, Pathogen Discovery Laboratory, Paris, France.
Isabelle PluDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Annick PrigentInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Lev StimmerInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Marie-Claude PotierInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Anne-Geneviève MarcelinDepartment of Virology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, INSERM 1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Paris, France.
Benoît DelatourInstitut du Cerveau - Paris Brain Institute - ICM, Inserm U1127, CNRS UMR7225, APHP, Sorbonne University, Pitié-Salpêtrière Hospital, Paris, France.
Charles DuyckaertsDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Danielle SeilheanDepartment of Neuropathology, Pitié-Salpêtrière Hospital, AP-HP Sorbonne University, Paris, France.
Centre National de la Recherche Scientifique · FRInserm · FRSorbonne Université · FRInstitut Pasteur · FRUniversité Paris-Saclay · FRAssistance Publique – Hôpitaux de Paris · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In a neuropathological series of 20 COVID-19 cases, we analyzed six cases (three biopsies and three autopsies) with multiple foci predominantly affecting the white matter as shown by MRI. The cases presented with microhemorrhages evocative of small artery diseases. This COVID-19 associated cerebral microangiopathy (CCM) was characterized by perivascular changes: arterioles were surrounded by vacuolized tissue, clustered macrophages, large axonal swellings and a crown arrangement of aquaporin-4 immunoreactivity. There was evidence of blood-brain-barrier leakage. Fibrinoid necrosis, vascular occlusion, perivascular cuffing and demyelination were absent. While no viral particle or viral RNA was found in the brain, the SARS-CoV-2 spike protein was detected in the Golgi apparatus of brain endothelial cells where it closely associated with furin, a host protease known to play a key role in virus replication. Endothelial cells in culture were not permissive to SARS-CoV-2 replication. The distribution of the spike protein in brain endothelial cells differed from that observed in pneumocytes. In the latter, the diffuse cytoplasmic labeling suggested a complete replication cycle with viral release, notably through the lysosomal pathway. In contrast, in cerebral endothelial cells the excretion cycle was blocked in the Golgi apparatus. Interruption of the excretion cycle could explain the difficulty of SARS-CoV-2 to infect endothelial cells

Indexed as

Blood-brain barrier (BBB)COVID-19FurinMicroangiopathyNeuro-Covid

Identifiers

PMID37283933
PMCPMC10240951
OpenAlexW4379601080

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.