Evidence map›Paper›PMID 37288664›Full record

ArticleJCI insight2023

Loss of ZNF148 enhances insulin secretion in human pancreatic β cells.

Eleonora de Klerk, Yini Xiao, Christopher H Emfinger, Mark P Keller, David I Berrios, Valentina Loconte, Axel A Ekman, Kate L White, Rebecca L Cardone, Richard G Kibbey and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JCI insight, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Eleonora de KlerkUCSF Diabetes Center, UCSF, San Francisco, California, USA.
Yini XiaoUCSF Diabetes Center, UCSF, San Francisco, California, USA.
Christopher H EmfingerDepartment of Biochemistry, University of Wisconsin-Madison, DeLuca Biochemistry Laboratories, Madison, Wisconsin, USA.
Mark P KellerDepartment of Biochemistry, University of Wisconsin-Madison, DeLuca Biochemistry Laboratories, Madison, Wisconsin, USA.
David I BerriosUCSF Diabetes Center, UCSF, San Francisco, California, USA.
Valentina LoconteDepartment of Anatomy, School of Medicine, UCSF, San Francisco, California, USA.
Axel A EkmanNational Center for X-ray Tomography, Advanced Light Source, Berkeley, California, USA.
Kate L WhiteMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Rebecca L CardoneDepartment of Internal Medicine (Endocrinology), Yale University, New Haven, Connecticut, USA.
Richard G KibbeyDepartment of Internal Medicine (Endocrinology), Yale University, New Haven, Connecticut, USA.
Alan D AttieDepartments of Biochemistry, Chemistry, and Medicine, University of Wisconsin-Madison, DeLuca Biochemistry Laboratories, Madison, Wisconsin, USA.
Matthias HebrokUCSF Diabetes Center, UCSF, San Francisco, California, USA.
University of Wisconsin System · USLawrence Berkeley National Laboratory · USYale University · USAdvanced Light Source

Funding

TrainingP41GM103445 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LARABELL, CAROLYN A · 2012 to 2019
$8.2M
The Diversity Outbred Diabetes ProjectR01DK101573 · NIDDK · UNIVERSITY OF WISCONSIN-MADISON · PI Alan D Attie · 2014 to 2026
$5.3M
User Training and OutreachP30GM138441 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LARABELL, CAROLYN A · 2020 to 2023
$3.7M
Regulation of beta cell identity and dedifferentiationR01DK105831 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HEBROK, MATTHIAS · 2015 to 2022
$2.9M
NIDDK NIH HHS R01 DK101573NIDDK NIH HHS R01 DK105831NIGMS NIH HHS P30 GM138441NIGMS NIH HHS P41 GM103445
6 · The paper itself

Abstract

Insulin secretion from pancreatic β cells is essential to the maintenance of glucose homeostasis. Defects in this process result in diabetes. Identifying genetic regulators that impair insulin secretion is crucial for the identification of novel therapeutic targets. Here, we show that reduction of ZNF148 in human islets, and its deletion in stem cell-derived β cells (SC-β cells), enhances insulin secretion. Transcriptomics of ZNF148-deficient SC-β cells identifies increased expression of annexin and S100 genes whose proteins form tetrameric complexes involved in regulation of insulin vesicle trafficking and exocytosis. ZNF148 in SC-β cells prevents translocation of annexin A2 from the nucleus to its functional place at the cell membrane via direct repression of S100A16 expression. These findings point to ZNF148 as a regulator of annexin-S100 complexes in human β cells and suggest that suppression of ZNF148 may provide a novel therapeutic strategy to enhance insulin secretion.

Indexed as

Insulin-Secreting CellsDNA-Binding ProteinsExocytosisGlucoseHumansInsulinInsulin SecretionTranscription FactorsDNA-Binding ProteinsGlucoseInsulinTranscription FactorsZNF148 protein, humanDiabetesEmbryonic stem cellsIslet cellsMetabolismStem cells

Identifiers

PMID37288664
PMCPMC10393241
OpenAlexW4379745027

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.