Evidence map›Paper›PMID 37310900›Full record

ArticleTissue engineering. Part C, Methods2023

Isolation and Characterization of Porcine Endocardial Endothelial Cells.

Kathleen N Brown, Hong Kim T Phan, Elysa L Jui, Marci K Kang, Jennifer P Connell, Sundeep G Keswani, K Jane Grande-Allen

Open access · greenAbstract read
In one paragraph

Article in Tissue engineering. Part C, Methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Kathleen N BrownDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Hong Kim T PhanDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Elysa L JuiDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Marci K KangDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Jennifer P ConnellDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Sundeep G KeswaniDivision of Pediatric Surgery, Department of Surgery, Texas Children's Hospital, Houston, Texas, USA.
K Jane Grande-AllenDepartment of Bioengineering, Rice University, Houston, Texas, USA.
Rice University · USBaylor College of Medicine · US

Funding

Molecular Physiology of the Cardiovascular SystemT32HL007676 · NHLBI · BAYLOR COLLEGE OF MEDICINE · PI HAMILTON, SUSAN L · 1989 to 2020
$7.9M
Differential Shear Forces on Endocardial Endothelial Cells Regulate a Fibrotic Spectrum in the Left Ventricular Outflow TractR01HL140305 · NHLBI · RICE UNIVERSITY · PI GRANDE-ALLEN, KATHRYN JANE, KESWANI, SUNDEEP G · 2018 to 2021
$2.2M
NHLBI NIH HHS R01 HL140305NHLBI NIH HHS T32 HL007676
6 · The paper itself

Abstract

The heart contains diverse endothelial cell types. We sought to characterize the endocardial endothelial cells (EECs), which line the chambers of the heart. EECs are relatively understudied, yet their dysregulation can lead to various cardiac pathologies. Due to the lack of commercial availability of these cells, we reported our protocol for isolating EECs from porcine hearts and for establishing an EEC population through cell sorting. In addition, we compared the EEC phenotype and fundamental behaviors to a well-studied endothelial cell line, human umbilical vein endothelial cells (HUVECs). The EECs stained positively for classic phenotypic markers such as CD31, von Willebrand Factor, and vascular endothelial (VE) cadherin. The EECs proliferated more quickly than HUVECs at 48 h (1310 ± 251 cells vs. 597 ± 130 cells,

Indexed as

EndocardiumHeartAnimalsCells, CulturedCell SeparationEndothelium, VascularHumansHuman Umbilical Vein Endothelial CellsSwinebehaviorcell isolationendocardialendothelial cellsphenotype

Identifiers

PMID37310900
PMCPMC10442675
OpenAlexW4380433768

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.