Evidence map›Paper›PMID 37337280›Full record

ArticleBMC research notes2023

Analysis of Notch1 signaling in mammalian sperm development.

Naoto Sambe, Masaharu Yoshihara, Teppei Nishino, Ryosuke Sugiura, Takahiro Nakayama, Chandra Louis, Satoru Takahashi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC research notes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 75% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Naoto SambeCollege of Medicine, School of Medicine and Health Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.ORCID http://orcid.org/0000-0002-8319-8301
Masaharu YoshiharaPhD Program in Humanics, School of Integrative and Global Majors, University of Tsukuba, 1- 1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan. yoshihara.masahar.ly@alumni.tsukuba.ac.jp.ORCID http://orcid.org/0000-0002-0212-0909
Teppei NishinoDepartment of Anatomy and Embryology, Institute of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.ORCID http://orcid.org/0000-0003-2544-4639
Ryosuke SugiuraCollege of Medicine, School of Medicine and Health Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.ORCID http://orcid.org/0000-0003-4175-4987
Takahiro NakayamaCollege of Medicine, School of Medicine and Health Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.ORCID http://orcid.org/0000-0002-4023-4560
Chandra LouisPhD Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8577, Japan.
Satoru TakahashiDepartment of Anatomy and Embryology, Institute of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.ORCID http://orcid.org/0000-0002-8540-7760
University of Tsukuba · JP

Funding

Japan Society for the Promotion of Science JP22K20734Ministry of Education, Culture, Sports, Science and Technology PhD Program in Humanics (Doctoral Program for World-leading Innovative and Smart Education)YOKOYAMA Foundation for Clinical Pharmacology YRY-2207
6 · The paper itself

Abstract

objectiveA mammalian Delta-Notch signaling component, Notch1, has been suggested for its expression during the normal sperm development although its conditional deletion caused no apparent abnormalities. Since we established our original transgenic mouse system that enabled labeling of past and ongoing Notch1 signaling at a cellular level, we tried to validate that observation in vivo. Our transgenic mouse system used Cre/loxP system to induce tandem dsRed expression upon Notch1 signaling.

resultsTo our surprise, we were unable to observe tandem dsRed expression in the seminiferous tubules where the sperms developed. In addition, tandem dsRed expression was lacking in the somatic cells of the next generation in our transgenic mouse system, suggesting that sperms received no Notch1 signaling during their development. To validate this result, we conducted re-analysis of four single-cell RNA-seq datasets from mouse and human testes and showed that Notch1 expression was little in the sperm cell lineage. Collectively, our results posed a question into the involvement of Notch1 in the normal sperm development although this observation may help the interpretation of the previous result that Notch1 conditional deletion caused no apparent abnormalities in murine spermatogenesis.

Indexed as

Receptor, Notch1TestisAnimalsHumansMaleMiceMice, TransgenicSemenSpermatozoaReceptor, Notch1Cre/loxP systemDelta-Notch signaling pathwayGal4/UAS systemSingle-cell RNA-seqTransgenic mouse

Identifiers

PMID37337280
PMCPMC10280896
OpenAlexW4381189978

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.