Evidence map›Paper›PMID 37352104›Full record

ArticleSTAR protocols2023

Protocol for extracting live blastoderm cells from embryos of annual killifish.

Sebastian Vásquez-Sepúlveda, Nestor Guerrero, Carmen Gloria Lemus, Margarita Meynard, Mauricio Cerda, Miguel Luis Concha, Cristina Bertocchi, Andrea Ravasio

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Sebastian Vásquez-SepúlvedaLaboratory for Mechanobiology of Transforming Systems, Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile; Laboratory for Molecular Mechanics of Cell Adhesion, Department of Physiology Pontificia Universidad Católica de Chile, Santiago 8320165, Chile.
Nestor GuerreroIntegrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.
Carmen Gloria LemusIntegrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.
Margarita MeynardIntegrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile; Center for Geroscience, Brain Health and Metabolism, Santiago 8380453, Chile.
Mauricio CerdaIntegrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile.
Miguel Luis ConchaIntegrative Biology Program, Institute of Biomedical Sciences, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile; Biomedical Neuroscience Institute, Santiago 8380453, Chile; Center for Geroscience, Brain Health and Metabolism, Santiago 8380453, Chile.
Cristina BertocchiLaboratory for Molecular Mechanics of Cell Adhesion, Department of Physiology Pontificia Universidad Católica de Chile, Santiago 8320165, Chile; Graduate School of Engineering Science, Osaka University, Osaka 565-0871, Japan. Electronic address: cbertocchi@bio.puc.cl.
Andrea RavasioLaboratory for Mechanobiology of Transforming Systems, Institute for Biological and Medical Engineering, Schools of Engineering, Medicine and Biological Sciences, Pontificia Universidad Católica de Chile, Santiago 7820436, Chile. Electronic address: ravasio@gmail.com.
University of Chile · CLPontificia Universidad Católica de Chile · CLInstituto de Neurociencia Biomédica · CL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The implementation of in vitro approaches using undifferentiated embryonic cells from annual killifish to complement existing in vivo developmental studies has been hindered by a lack of efficient isolation techniques. Here, we present a protocol to isolate annual killifish blastoderm cells, at the epiboly and early dispersion phase, from embryos. We describe steps for hair removal, embryo cleaning, dechorionation, and cell purification. This protocol may also be used to develop strategies to isolate cells from embryos presenting similar challenges.

Indexed as

BlastodermEmbryo, NonmammalianAnimalsMorphogenesisCell isolationDevelopmental biologyModel Organisms

Identifiers

PMID37352104
PMCPMC10320274
OpenAlexW4381682940

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.