Evidence map›Paper›PMID 37389242›Full record

ArticleWorld journal of gastroenterology2023

Study of the roles of caspase-3 and nuclear factor kappa B in myenteric neurons in a P2X7 receptor knockout mouse model of ulcerative colitis.

Henrique Inhauser Riceti Magalhães, Felipe Alexandre Machado, Roberta Figueiroa Souza, Marcos Antônio Ferreira Caetano, Vanessa Ribeiro Figliuolo, Robson Coutinho-Silva, Patricia Castelucci

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In one paragraph

Article in World journal of gastroenterology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Enteric nervous system as a therapeutic target in gastrointestinal disorders.World journal of gastrointestinal pharmacology and therapeutics · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Henrique Inhauser Riceti MagalhãesDepartment of Surgery, School of Veterinary Medicine and Animal Sciences, University of São Paulo, São Paulo 05508-270, Brazil.
Felipe Alexandre MachadoDepartment of Anatomy, University of São Paulo, São Paulo 05508-000, Brazil.
Roberta Figueiroa SouzaDepartment of Anatomy, University of São Paulo, São Paulo 05508-000, Brazil.
Marcos Antônio Ferreira CaetanoDepartment of Anatomy, University of São Paulo, São Paulo 05508-000, Brazil.
Vanessa Ribeiro FigliuoloBiophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Robson Coutinho-SilvaBiophysics Institute Carlos Chagas Filho, Federal University of Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.
Patricia CastelucciDepartment of Anatomy, University of São Paulo, São Paulo 05508-000, Brazil. pcastel@usp.br.
Universidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe literature indicates that the enteric nervous system is affected in inflammatory bowel diseases (IBDs) and that the P2X7 receptor triggers neuronal death. However, the mechanism by which enteric neurons are lost in IBDs is unknown.

aimTo study the role of the caspase-3 and nuclear factor kappa B (NF-κB) pathways in myenteric neurons in a P2X7 receptor knockout (KO) mouse model of IBDs.

methodsForty male wild-type (WT) C57BL/6 and P2X7 receptor KO mice were euthanized 24 h or 4 d after colitis induction by 2,4,6-trinitrobenzene sulfonic acid (colitis group). Mice in the sham groups were injected with vehicle. The mice were divided into eight groups (

resultsCells double labeled for calretinin and P2X7 receptor, cleaved caspase-3, total caspase-3, phospho-NF-κB, or total NF-κB were observed in the WT colitis 24 h and 4 d groups. The number of calretinin-ir neurons per ganglion was decreased in the WT colitis 24 h and 4 d groups compared to the WT sham 24 h and 4 d groups, respectively (2.10 ± 0.13

conclusionUlcerative colitis affects myenteric neurons in WT mice but has a weaker effect in P2X7 receptor KO mice, and neuronal death may be associated with P2X7 receptor-mediated caspase-3 activation. The P2X7 receptor can be a therapeutic target for IBDs.

Indexed as

ColitisColitis, UlcerativeInflammatory Bowel DiseasesAnimalsCalbindin 2Caspase 3MaleMiceMice, Inbred C57BLNF-kappa BReceptors, Purinergic P2X7Calbindin 2Casp3 protein, mouseCaspase 3NF-kappa BP2rx7 protein, mouseReceptors, Purinergic P2X7Cell deathEnteric nervous systemGastroenterologyInflammatory bowel diseasesP2X7 receptorPurinergic signaling

Identifiers

PMID37389242
PMCPMC10303518
OpenAlexW4379531511

What Socratic holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.