Evidence map›Paper›PMID 37408215›Full record

ArticleCells2023

Conversion of Unmodified Stem Cells to Pacemaker Cells by Overexpression of Key Developmental Genes.

Tahereh Karimi, Zhizhong Pan, Vladimir N Potaman, Eckhard U Alt

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 1 country.

Tahereh KarimiHeart and Vascular Institute, Department of Medicine, Tulane University Health Science Center, 1430 Tulane Ave, New Orleans, LA 70112, USA.
Zhizhong PanUniversity of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
Vladimir N PotamanAlliance of Cardiovascular Researchers, 2211 Augusta Dr #10, Houston, TX 77057, USA.
Eckhard U AltHeart and Vascular Institute, Department of Medicine, Tulane University Health Science Center, 1430 Tulane Ave, New Orleans, LA 70112, USA.
The University of Texas MD Anderson Cancer Center · USTulane University · USUniversity of South Dakota · US

Funding

Dnmt3a as an epigenetic target for chronic pain treatmentRF1NS113256 · NINDS · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI PAN, ZHIZHONG Z. · 2019 to 2019
$2.7M
NINDS NIH HHS RF1 NS113256
6 · The paper itself

Abstract

Arrhythmias of the heart are currently treated by implanting electronic pacemakers and defibrillators. Unmodified adipose tissue-derived stem cells (ASCs) have the potential to differentiate into all three germ layers but have not yet been tested for the generation of pacemaker and Purkinje cells. We investigated if-based on overexpression of dominant conduction cell-specific genes in ASCs-biological pacemaker cells could be induced. Here we show that by overexpression of certain genes that are active during the natural development of the conduction system, the differentiation of ASCs to pacemaker and Purkinje-like cells is feasible. Our study revealed that the most effective procedure consisted of short-term upregulation of gene combinations SHOX2-TBX5-HCN2, and to a lesser extent SHOX2-TBX3-HCN2. Single-gene expression protocols were ineffective. Future clinical implantation of such pacemaker and Purkinje cells, derived from unmodified ASCs of the same patient, could open up new horizons for the treatment of arrythmias.

Indexed as

Hyperpolarization-Activated Cyclic Nucleotide-Gated ChannelsMyocytes, CardiacGenes, DevelopmentalHeart Conduction SystemHumansStem CellsHyperpolarization-Activated Cyclic Nucleotide-Gated Channelsadipose-derived stem cellsion channelspacemaker cellstranscription factors

Identifiers

PMID37408215
PMCPMC10216671
OpenAlexW4376611876

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.