ArticleBMC plant biology2023
Genome-wide systematic characterization of the NRT2 gene family and its expression profile in wheat (Triticum aestivum L.) during plant growth and in response to nitrate deficiency.
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 20 citations in OpenAlex.
- Characterisation and expression profiles of the NPF gene family in Cannabis sativa L. under low nitrogen.BMC plant biology · 2026Article
- Combined transcriptome and metabolome analysis reveals nitrogen-responsive genes and metabolites in sugar beet (Frontiers in plant science · 2026Article
- Functional Insights intoPlants (Basel, Switzerland) · 2025Article
- Hyperspectral imaging of grains uncovers the genetic architecture of nitrogen response of development in bread wheat.Molecular breeding : new strategies in plant improvement · 2025Article
- Genome-Wide Identification, Phylogeny and Expression Analysis of the Magnesium Release Gene Family in Wheat (Current issues in molecular biology · 2025Article
- Wheat COBRA-like GeneInternational journal of molecular sciences · 2025Article
- Stable pleotropic loci controlling the accumulation of multiple nutritional elements in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Harnessing the Potential of CRISPR/Cas in Targeted Alfalfa Improvement for Stress Resilience.International journal of molecular sciences · 2025Review
- Natural variation in ZmNRT2.5 modulates husk leaf width and promotes seed protein content in maize.Plant biotechnology journal · 2025Article
- Plasticity of Root System Architecture and Whole Transcriptome Responses Underlying Nitrogen Deficiency Tolerance Conferred by a Wild Emmer Wheat QTL.Plant, cell & environment · 2025Article
- Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family under phytohormones and abiotic stresses in alfalfa (Medicago sativa).Scientific reports · 2024Article
- Functional analyses of the NRT2 family of nitrate transporters inFrontiers in plant science · 2024Review
- The Functional Diversity of the High-Affinity Nitrate Transporter Gene Family in Hexaploid Wheat: Insights from Distinct Expression Profiles.International journal of molecular sciences · 2023Article
- Exploring the genetic landscape of nitrogen uptake in durum wheat: genome-wide characterization and expression profiling of NPF and NRT2 gene families.Frontiers in plant science · 2023Article
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
backgroundWheat (Triticum aestivum L.) is a major cereal crop that is grown worldwide, and it is highly dependent on sufficient N supply. The molecular mechanisms associated with nitrate uptake and assimilation are still poorly understood in wheat. In plants, NRT2 family proteins play a crucial role in NO
resultsIn this study, a comprehensive analysis of wheat TaNRT2 genes was conducted using bioinformatics and molecular biology methods, and 49 TaNRT2 genes were identified. A phylogenetic analysis clustered the TaNRT2 genes into three clades. The genes that clustered on the same phylogenetic branch had similar gene structures and nitrate assimilation functions. The identified genes were further mapped onto the 13 wheat chromosomes, and the results showed that a large duplication event had occurred on chromosome 6. To explore the TaNRT2 gene expression profiles in wheat, we performed transcriptome sequencing after low nitrate treatment for three days. Transcriptome analysis revealed the expression levels of all TaNRT2 genes in shoots and roots, and based on the expression profiles, three highly expressed genes (TaNRT2-6A.2, TaNRT2-6A.6, and TaNRT2-6B.4) were selected for qPCR analysis in two different wheat cultivars ('Mianmai367' and 'Nanmai660') under nitrate-limited and normal conditions. All three genes were upregulated under nitrate-limited conditions and highly expressed in the high nitrogen use efficiency (NUE) wheat 'Mianmai367' under low nitrate conditions.
conclusionWe systematically identified 49 NRT2 genes in wheat and analysed the transcript levels of all TaNRT2s under nitrate deficient conditions and over the whole growth period. The results suggest that these genes play important roles in nitrate absorption, distribution, and accumulation. This study provides valuable information and key candidate genes for further studies on the function of TaNRT2s in wheat.
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