ArticleNature communications2023
A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
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- RATEX: A Scalable RNA-Based Platform for Logical and Multi-Layered Cellular Programming.Angewandte Chemie (International ed. in English) · 2026Article
- Construction and analysis of a cell factory for terpenoid biosynthesis inSynthetic and systems biotechnology · 2026Article
- Multi-layered engineering of Aspergillus terreus enhances biosynthesis of the plant-derived fungicide physcion.Microbial cell factories · 2025Article
- A hidden intrinsic ability of bicistronic expression based on a novel translation reinitiation mechanism in yeast.Nucleic acids research · 2025Article
- Engineering artificial cross-species promoters with different transcriptional strengths.Synthetic and systems biotechnology · 2025Article
- Developing polycistronic expression tool inSynthetic and systems biotechnology · 2025Article
- Utilizing the Fungal Bicistronic System for Multi-Gene Expression to Generate Insect-Resistant and Herbicide-Tolerant Maize.International journal of molecular sciences · 2024Article
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- In silico characterization of Rhodotorula toruloides ELO-like elongases and production of very-long-chain fatty acids by expressing Rtelo2, RtKCR, RtHCD, and RtECR through IRES-mediated bicistrons.World journal of microbiology & biotechnology · 2024Article
- Current status of molecular rice breeding for durable and broad-spectrum resistance to major diseases and insect pests.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Copper-Induced In Vivo Gene Amplification in Budding Yeast.Biodesign research · 2024Article
- The mycoremediation potential of the armillarioids: a comparative genomics analysis.Frontiers in bioengineering and biotechnology · 2023Article
Corrections and comments
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Authors and funding
15 authors at 8 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology requires efficient systems that support the well-coordinated co-expression of multiple genes. Here, we discover a 9-bp nucleotide sequence that enables efficient polycistronic gene expression in yeasts and filamentous fungi. Coupling polycistronic expression to multiplexed, markerless, CRISPR/Cas9-based genome editing, we develop a strategy termed HACKing (Highly efficient and Accessible system by CracKing genes into the genome) for the assembly of multigene pathways. HACKing allows the expression level of each enzyme to be precalibrated by linking their translation to those of host proteins with predetermined abundances under the desired fermentation conditions. We validate HACKing by rapidly constructing highly efficient Saccharomyces cerevisiae cell factories that express 13 biosynthetic genes, and produce model endogenous (1,090.41 ± 80.92 mg L
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.