Evidence map›Paper›PMID 37460896›Full record

ArticleNature structural & molecular biology2023

Sperm chromatin structure and reproductive fitness are altered by substitution of a single amino acid in mouse protamine 1.

Lindsay Moritz, Samantha B Schon, Mashiat Rabbani, Yi Sheng, Ritvija Agrawal, Juniper Glass-Klaiber, Caleb Sultan, Jeannie M Camarillo, Jourdan Clements, Michael R Baldwin and 11 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature structural & molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Protamine lacunae preserve the paternal chromatin landscape in sperm.bioRxiv : the preprint server for biology · 2025
    Article
  7. Rapid protamine evolution suppresses meiotic drive inbioRxiv : the preprint server for biology · 2025
    Article
  8. Article
  9. Article
  10. Review
  11. Article
  12. Review
  13. Article
  14. The protamines of the noble false widow spiderbioRxiv : the preprint server for biology · 2024
    Article
  15. Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 6 institutions in 1 country.

Lindsay Moritz *Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI, USA.
Samantha B Schon *Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA.
Mashiat RabbaniDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, USA.
Yi ShengDepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Ritvija AgrawalDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
Juniper Glass-KlaiberDepartment of Biochemistry and Molecular Biotechnology, University of Massachusetts Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0001-9212-725X
Caleb SultanDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, USA.
Jeannie M CamarilloDepartments of Chemistry, Molecular Biosciences, and the National Resource for Translational and Developmental Proteomics, Northwestern University, Evanston, IL, USA.
Jourdan ClementsDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, USA.
Michael R BaldwinDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.
Adam G DiehlDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Alan P BoyleDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0002-2081-1105
Patrick J O'BrienDepartment of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0001-7853-8626
Kaushik RagunathanDepartment of Biology, Brandeis University, Waltham, MA, USA.
Yueh-Chiang HuDivision of Developmental Biology, Cincinnati Children's Hospital Medical Center, Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.ORCID http://orcid.org/0000-0002-7992-0525
Neil L KelleherDepartments of Chemistry, Molecular Biosciences, and the National Resource for Translational and Developmental Proteomics, Northwestern University, Evanston, IL, USA.ORCID http://orcid.org/0000-0002-8815-3372
Jayakrishnan NandakumarDepartment of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA.ORCID http://orcid.org/0000-0001-9146-2785
Jun Z LiDepartment of Human Genetics, University of Michigan, Ann Arbor, MI, USA.
Kyle E OrwigDepartment of Obstetrics, Gynecology and Reproductive Sciences, Magee Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0002-7952-8419
Sy ReddingDepartment of Biochemistry and Molecular Biotechnology, University of Massachusetts Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0003-3463-7985
Saher Sue HammoudCellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI, USA. hammou@med.umich.edu.ORCID http://orcid.org/0000-0002-5040-6370
University of Michigan · USNorthwestern University · USUniversity of Massachusetts Chan Medical School · USUniversity of Pittsburgh · USBrandeis University · USCincinnati Children's Hospital Medical Center · US

Funding

TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6M
CELLULAR AND MOLECULAR BIOLOGY AT MICHIGANT32GM007315 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI PUTHENVEEDU, MANOJKUMAR A · 1985 to 2021
$12.8M
MOLECULAR GENETICS OF BASIC CELL FUNCTIONST32GM007310 · NIGMS · TUFTS UNIVERSITY BOSTON · PI CAMILLI, ANDREW · 1987 to 2020
$3.9M
University of Michigan WRHR Career Development ProgramK12HD065257 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FENNER, DEE ELLEN · 2010 to 2019
$3.4M
Heterochromatin dynamics during inheritance and adaptationR35GM137832 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Kaushik Ragunathan · 2020 to 2026
$3.2M
The role of ZCWPW1 in meiosisR01GM148028 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Francesca Cole, Saher Sue Hammoud · 2023 to 2026
$2.4M
Contributions of Sperm Chromatin to development: A Myth or Reality?DP2HD091949 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2016 to 2016
$2.3M
Molecular functions of the shelterin component ACD/TPP1 in somatic stem cells and tissue homeostasisR01AG050509 · NIA · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI KEEGAN, CATHERINE ELIZABETH, MAILLARD, IVAN · 2015 to 2019
$2.3M
Physical determinants of DNA recognition and genome organization in crowded environmentsR35GM147477 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI Sy Redding · 2022 to 2026
$2.1M
Mechanisms of sperm chromatin remodeling in vivo and in vitroR01HD113274 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Saher Sue Hammoud · 2023 to 2026
$2.0M
Sperm Chromatin: Implications on organismal development and fertilityR01HD104680 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HAMMOUD, SAHER SUE · 2021 to 2024
$1.8M
Mechanisms of Base Excision DNA RepairR35GM149546 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Patrick J O'Brien · 2023 to 2026
$1.8M
NIA NIH HHS R01 AG050509NICHD NIH HHS DP2 HD091949NICHD NIH HHS K12 HD065257NICHD NIH HHS R01 HD104680NICHD NIH HHS R01 HD113274NICHD NIH HHS R03 HD101501NICHD NIH HHS R21 HD090371NICHD NIH HHS R21 HD113812NIGMS NIH HHS P41 GM108569NIGMS NIH HHS R01 GM120094NIGMS NIH HHS R01 GM148028NIGMS NIH HHS R35 GM137832NIGMS NIH HHS R35 GM147477NIGMS NIH HHS R35 GM149546NIGMS NIH HHS T32 GM007310NIGMS NIH HHS T32 GM007315
6 · The paper itself

Abstract

Conventional dogma presumes that protamine-mediated DNA compaction in sperm is achieved by electrostatic interactions between DNA and the arginine-rich core of protamines. Phylogenetic analysis reveals several non-arginine residues conserved within, but not across species. The significance of these residues and their post-translational modifications are poorly understood. Here, we investigated the role of K49, a rodent-specific lysine residue in protamine 1 (P1) that is acetylated early in spermiogenesis and retained in sperm. In sperm, alanine substitution (P1(K49A)) decreases sperm motility and male fertility-defects that are not rescued by arginine substitution (P1(K49R)). In zygotes, P1(K49A) leads to premature male pronuclear decompaction, altered DNA replication, and embryonic arrest. In vitro, P1(K49A) decreases protamine-DNA binding and alters DNA compaction and decompaction kinetics. Hence, a single amino acid substitution outside the P1 arginine core is sufficient to profoundly alter protein function and developmental outcomes, suggesting that protamine non-arginine residues are essential for reproductive fitness.

Indexed as

Amino AcidsGenetic FitnessAnimalsArginineChromatinDNAMaleMicePhylogenyProtaminesSemenSpermatozoaSperm MotilityAmino AcidsArginineChromatinDNAPrm1 protein, mouseProtamines

Identifiers

PMID37460896
PMCPMC10833441
OpenAlexW4384522319

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.