Evidence mapPaperPMID 37468947Full record

ArticleBMC oral health2023

Effect of an low-energy Nd: YAG laser on periodontal ligament stem cell homing through the SDF-1/CXCR4 signaling pathway.

Nan Wu, Jianing Song, Xin Liu, Xiangtao Ma, Xiaoman Guo, Taohong Liu, Mingxuan Wu

Open access · goldAbstract read
In one paragraph

Article in BMC oral health, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

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  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Nan WuHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Jianing SongHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Xin LiuHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Xiangtao MaHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Xiaoman GuoHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Taohong LiuHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China.
Mingxuan WuHebei Key Laboratory of Stomatology, Department of Periodontology (II), Hebei Clinical Research Center for Oral Diseases, School and Hospital of Stomatology, Hebei Medical University, Zhongshan East Road 383, Shijiazhuang, 050017, Hebei, People's Republic of China. 15608758@qq.com.
Hebei Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe key to the success of endogenous regeneration is to improve the homing rate of stem cells, and low-energy laser is an effective auxiliary means to promote cell migration and proliferation. The purpose of this study was to observe whether low-energy neodymium (Nd: YAG) laser with appropriate parameters can affect the proliferation and migration of periodontal ligament stem cells (PDLSCs) through SDF-1/CXCR4 pathway.

methodsh PDLSCs were cultured and identified. CCK8 assay was used to detect the proliferation of h PDLSCs after different power (0, 0.25, 0.5, 1, and 1.5 W) Nd: YAG laser (MSP, 10 Hz, 30 s, 300 μ m) irradiation at 2th, 3rd,5th, and 7th days, and the optimal laser irradiation parameters were selected for subsequent experiments. Then, the cells were categorized into five groups: control group (C), SDF-1 group (S), AMD3100 group (A), Nd: YAG laser irradiation group (N), and Nd: YAG laser irradiation + AMD3100 group (N + A). the migration of h PDLSCs was observed using Transwell, and the SDF-1 expression was evaluated using ELISA andRT-PCR. The SPSS Statistics 21.0 software was used for statistical analysis.

resultsThe fibroblasts cultured were identified as h PDLSCs. Compared with the C, when the power was 1 W, the proliferation rate of h PDLSCs was accelerated (P < 0.05). When the power was 1.5 W, the proliferation rate decreased (P < 0.05). When the power was 0.25 and 0.5 W, no statistically significant difference in the proliferation rate was observed (P > 0.05). The number of cell perforations values as follows: C (956.5 ± 51.74), A (981.5 ± 21.15), S (1253 ± 87.21), N (1336 ± 48.54), and N + A (1044 ± 22.13), that increased significantly in group N (P < 0.05), but decreased in group N + A (P < 0.05). The level of SDF-1 and the expression level of SDF-1 mRNA in groups N and N + A was higher than that in group C (P < 0.05) but lower than that in group A (P < 0.05).

conclusionsNd: YAG laser irradiation with appropriate parameters provides a new method for endogenous regeneration of periodontal tissue. SDF-1/CXCR4 signaling pathway may be the mechanism of LLLT promoting periodontal regeneration.

Indexed as

Cell MovementLasers, Solid-StatePeriodontal LigamentStem CellsBenzylaminesCyclamsHumansReceptors, CXCR4BenzylaminesCXCR4 protein, humanCyclamsplerixaforReceptors, CXCR4CXCR4 signaling pathwayEndogenous periodontal regenerationLow level laser therapyPeriodontal ligament stem cellsSDF-1

Identifiers

PMID37468947
PMCPMC10357748
OpenAlexW4384818742

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.