Evidence map›Paper›PMID 37468984›Full record

ArticleCell & bioscience2023

Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos.

Qianqi Zhu, Miaoying Tan, Chengniu Wang, Yufei Chen, Chenfei Wang, Junqi Zhang, Yijun Gu, Yuqi Guo, Jianpeng Han, Lei Li and 8 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell & bioscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 1 institution in 1 country.

Qianqi Zhu *Department of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Miaoying Tan *Department of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Chengniu Wang *Institute of Reproductive Medicine, Medical School of Nantong University, Nantong, 226001, China.
Yufei ChenInstitute of Reproductive Medicine, Medical School of Nantong University, Nantong, 226001, China.
Chenfei WangDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Junqi ZhangDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Yijun GuDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Yuqi GuoDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Jianpeng HanDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Lei LiDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Rongrong JiangDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Xudong FanDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Huimin XieDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Liang WangDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China.
Zhifeng GuResearch Center of Clinical Medicine, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China. guzf@ntu.edu.cn.
Dong LiuSchool of Life Science, Nantong Laboratory of Development and Diseases Second Affiliated Hospital Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226019, China. liudongtom@gmail.com.
Jianwu ShiInstitute of Reproductive Medicine, Medical School of Nantong University, Nantong, 226001, China. jwshi@ntu.edu.cn.
Xingmei FengDepartment of Stomatology, Affiliated Hospital of Nantong University, Medical School of, Nantong University, Nantong, 226001, China. xingmeifeng@ntu.edu.cn.ORCID http://orcid.org/0000-0002-2063-5885
Nantong University · CN

Funding

Funing Meite Dental Materials Facto ZZXM-20201008Funing Meite Dental Materials Facto ZZXM-20201009Jiangsu Yancheng Timstar Medical Technology Co., Lt HXKT20211014Jiangsu Yancheng Timstar Medical Technology Co., Lt HXKT20211018Nantong City Science and Technology Projects Funds MS12020030National Natural Science Foundation of China 2018YFA0801004National Natural Science Foundation of China 81870359National Natural Science Foundation of China 82001606National Natural Science Foundation of China 82071838Natural Science Foundation of Jiangsu Province BK20180048Natural Science Foundation of Jiangsu Province BRA2019278Natural Science Foundation of Nantong City JC2021081Wu Yingkai Foundation for Medical Research and Development HXKT20200019
6 · The paper itself

Abstract

backgroundThe temporomandibular joint (TMJ) is a complex joint consisting of the condyle, the temporal articular surface, and the articular disc. Functions such as mastication, swallowing and articulation are accomplished by the movements of the TMJ. To date, the TMJ has been studied more extensively, but the types of TMJ cells, their differentiation, and their interrelationship during growth and development are still unclear and the study of the TMJ is limited. The aim of this study was to establish a molecular cellular atlas of the human embryonic temporomandibular joint condyle (TMJC) by single-cell RNA sequencing, which will contribute to understanding and solving clinical problems.

resultsHuman embryos at 3 and 4 months of age are an important stage of TMJC development. We performed a comprehensive transcriptome analysis of TMJC tissue from human embryos at 3 and 4 months of age using single-cell RNA sequencing. A total of 16,624 cells were captured and the gene expression profiles of 15 cell clusters in human embryonic TMJC were determined, including 14 known cell types and one previously unknown cell type, "transition state cells (TSCs)". Immunofluorescence assays confirmed that TSCs are not the same cell cluster as mesenchymal stem cells (MSCs). Pseudotime trajectory and RNA velocity analysis revealed that MSCs transformed into TSCs, which further differentiated into osteoblasts, hypertrophic chondrocytes and tenocytes. In addition, chondrocytes (CYTL1

conclusionsOur study provides an atlas of differentiation stages of human embryonic TMJC tissue cells, which will contribute to an in-depth understanding of the pathophysiology of the TMJC tissue repair process and ultimately help to solve clinical problems.

Indexed as

Cellular transcriptomicsGene expressionHuman embryonic cellsTMJC

Identifiers

PMID37468984
PMCPMC10357633
OpenAlexW4384818690

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.