ArticlebioRxiv : the preprint server for biology2023
Validation of GCN5L1/BLOC1S1/BLOS1 Antibodies Using Knockout Cells and Tissue.
Paramesha Bugga, Michael W Stoner, Janet R Manning, Bellina A S Mushala, Dharendra Thapa, Iain Scott
Open access · greenAbstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
6 authors at 2 institutions in 1 country.
Paramesha BuggaVascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
Michael W StonerVascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
Janet R ManningVascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
Bellina A S MushalaVascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
Dharendra ThapaDepartment of Human Performance - Exercise Physiology, West Virginia University, Morgantown, WV 26506.
Iain ScottVascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261.
University of Pittsburgh · USWest Virginia University · US
Funding
Novel strategies to resolve metabolic defects in the diabetic heartR01HL147861 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCOTT, IAIN · 2020 to 2024
$2.4MFatty acid oxidation in female cardioprotectionR01HL156874 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCOTT, IAIN · 2022 to 2025
$2.2MRegulation of Fuel Utilization by Lysine Acetylation in the Failing HeartR01HL132917 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCOTT, IAIN · 2017 to 2020
$1.9MPredoctoral Training in Pharmacological Sciences (Resubmission)T32GM133332 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Tija C. Jacob, Francisco Jose Schopfer · 2020 to 2026
$1.8MInvestigating the novel role of acetylation in cardiac mitochondrial bioenergetics and function in the aging heartR00HL146905 · NHLBI · WEST VIRGINIA UNIVERSITY · PI THAPA, DHARENDRA · 2021 to 2023
$747kRegulation of Fuel Utilization by Lysine Acetylation in the Failing HeartR56HL132917 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCOTT, IAIN · 2016 to 2016
$469kInvestigating the novel role of acetylation in cardiac mitochondrial bioenergetics and function in the aging heartK99HL146905 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI THAPA, DHARENDRA · 2020 to 2021
$346kTargeting the hepatic adropin signaling pathway in obesityF31DK134089 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MUSHALA, BELLINA · 2023 to 2023
$43kNHLBI NIH HHS K99 HL146905NHLBI NIH HHS R00 HL146905NHLBI NIH HHS R01 HL132917NHLBI NIH HHS R01 HL147861NHLBI NIH HHS R01 HL156874NHLBI NIH HHS R56 HL132917NIDDK NIH HHS F31 DK134089NIGMS NIH HHS T32 GM133332
6 · The paper itselfAbstract
GCN5L1, also known as BLOC1S1 and BLOS1, is a small intracellular protein involved in a number of key biological processes. Over the last decade, GCN5L1 has been implicated in the regulation of protein lysine acetylation, energy metabolism, endo-lysosomal function, and cellular immune pathways. An increasing number of published papers have used commercially-available reagents to interrogate GCN5L1 function. However, in many cases these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or
Indexed as
AntibodyAutophagyBLOC1S1BLOS1GCN5L1LysosomeMitochondria
Identifiers
PMID37503156
PMCPMC10370191
OpenAlexW4385074503
What Socratic holds
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LicenceCC BY-NC-ND
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