ReviewAnimals : an open access journal from MDPI2023
An Overview on Assay Methods to Quantify ROS and Enzymatic Antioxidants in Erythrocytes and Spermatozoa of Small Domestic Ruminants.
Review in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Integration of Proteomics and Phenomics to Reveal Molecular Differences in High and Low Semen Quality in Donkeys.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Phytochemical and Preliminary Biological Evaluation ofPharmaceuticals (Basel, Switzerland) · 2026Article
- Microwave synthesis of ultrasmall gold nanoparticles, their γ-globulin interaction and subsequent effects in hepatocellular carcinoma cells.BMC chemistry · 2026Article
- The Role of Ethyl Acetate Fraction fromMolecules (Basel, Switzerland) · 2026Article
- Iron deficiency anemia perturbs erythrocyte morphology and may promote eryptosis.International journal of hematology · 2026Article
- Role of propolis and rosemary extract in improving mitochondrial efficiency and energy metabolism enzymes in late pregnancy Awassi sheep liver.Open veterinary journal · 2026Article
- Effect of Different Extenders on the Oxidative Status and Fertility of Sarda Ram Liquid Semen Stored at 15 °C.Antioxidants (Basel, Switzerland) · 2025Article
- Effect of PUFAs-ω3 and ω6 on oxidative stress of sheep erythrocytes.BMC veterinary research · 2025Article
- Antibacterial and Antiprotease activities of fermented barnyard millet (Food chemistry: X · 2025Article
- Polydopamine-based nano-protectant for prolonged boar semen preservation by eliminating ROS and regulating protein phosphorylation via D2DR-mediated cAMP/PKA signaling pathway.Journal of nanobiotechnology · 2025Article
- Comparative Aquatic Risk of Three Glyphosate-Based Herbicides Using Early-Stage Development ofJournal of toxicology · 2025Article
- Cannabidiol suppresses proliferation and induces cell death, autophagy and senescence in human cholangiocarcinoma cells via the PI3K/AKT/mTOR pathway.Journal of traditional and complementary medicine · 2024Article
- Combinatory Effect of Pequi Oil (Pharmaceutics · 2024Article
- Impact of Heat Stress on the Balance between Oxidative Markers and the Antioxidant Defence System in the Plasma of Mid-Lactating Modicana Dairy Cows.Animals : an open access journal from MDPI · 2024Article
- Advancements in Understanding and Enhancing Antioxidant-Mediated Sperm Cryopreservation in Small Ruminants: Challenges and Perspectives.Antioxidants (Basel, Switzerland) · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The present review aims to provide an overview of the assay methods for the quantification of ROS and principal enzymatic antioxidants as biomarkers of oxidative stress in erythrocytes and spermatozoa of small domestic ruminants. A complete literature search was carried out in PubMed, Scopus and the World Wide Web using relevant keywords and focusing on the last five years (2018-2023). Among spectrophotometry, fluorometry and chemiluminescence, the most widely used method for ROS assay is fluorometry, probably because it allows to simultaneously assay several ROS, using different probes, with greater economic advantages. Regarding intracellular antioxidant enzymes, recent literature reports only spectrophotometric methods, many of which use commercial kits. The use of a less sensitive but cheapest method is suitable because both erythrocytes and spermatozoa samples are highly concentrated in domestic ruminant species. All methods considered in this review have been found to be appropriate; in general, the differences are related to their costs and sensitivity. Quantification of ROS and enzymatic antioxidant activity in erythrocytes and spermatozoa may find application in the study of the welfare and health status of small domestic ruminants for monitoring livestock production.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.