Evidence map›Paper›PMID 37515350›Full record

ArticleJournal of biological rhythms2023

Roles for the

Briana M McKnight, Shannon Kang, Tam H Le, Mingxu Fang, Genelyn Carbonel, Esbeydi Rodriguez, Sutharsan Govindarajan, Nitsan Albocher-Kedem, Amanda L Tran, Nicholas R Duncan and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological rhythms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Circadian clock proteins KaiB and Rbp2 ofMicrobiology spectrum · 2026
    Article
  4. The RRM domain-containing protein Rbp3 interacts with ribosomes and the 3' ends of mRNAs encoding photosynthesis proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Briana M McKnightDepartment of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Shannon KangDepartment of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Tam H LeDepartment of Biological Sciences, California State University, Los Angeles, Los Angeles, CA, USA.
Mingxu FangCenter for Circadian Biology, University of California, San Diego, La Jolla, CA, USA.
Genelyn CarbonelDepartment of Biological Sciences, California State University, Los Angeles, Los Angeles, CA, USA.
Esbeydi RodriguezDepartment of Biological Sciences, California State University, Los Angeles, Los Angeles, CA, USA.
Sutharsan GovindarajanDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Nitsan Albocher-KedemDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Amanda L TranDepartment of Biological Sciences, California State University, Los Angeles, Los Angeles, CA, USA.
Nicholas R DuncanDepartment of Biological Sciences, California State University, Los Angeles, Los Angeles, CA, USA.ORCID 0000-0002-1032-4610
Orna Amster-ChoderDepartment of Microbiology and Molecular Genetics, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Susan S GoldenDepartment of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Susan E CohenCenter for Circadian Biology, University of California, San Diego, La Jolla, CA, USA.ORCID 0000-0003-1783-0011
California State University Los Angeles · USUniversity of California San Diego · USHebrew University of Jerusalem · IL

Funding

Undergraduate Summer Research Experience: Molecular and cellular mechanisms of circadian timekeeping in a prokaryote modelR35GM118290 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GOLDEN, SUSAN S · 2016 to 2025
$6.7M
NIGMS NIH HHS R35 GM118290
6 · The paper itself

Abstract

The cyanobacterial circadian oscillator, consisting of KaiA, KaiB, and KaiC proteins, drives global rhythms of gene expression and compaction of the chromosome and regulates the timing of cell division and natural transformation. While the KaiABC posttranslational oscillator can be reconstituted in vitro, the Kai-based oscillator is subject to several layers of regulation in vivo. Specifically, the oscillator proteins undergo changes in their subcellular localization patterns, where KaiA and KaiC are diffuse throughout the cell during the day and localized as a focus at or near the pole of the cell at night. Here, we report that the CI domain of KaiC, when in a hexameric state, is sufficient to target KaiC to the pole. Moreover, increased ATPase activity of KaiC correlates with enhanced polar localization. We identified proteins associated with KaiC in either a localized or diffuse state. We found that loss of Rbp2, found to be associated with localized KaiC, results in decreased incidence of KaiC localization and long-period circadian phenotypes. Rbp2 is an RNA-binding protein, and it appears that RNA-binding activity of Rbp2 is required to execute clock functions. These findings uncover previously unrecognized roles for Rbp2 in regulating the circadian clock and suggest that the proper localization of KaiC is required for a fully functional clock in vivo.

Indexed as

Circadian ClocksSynechococcusBacterial ProteinsCircadian RhythmCircadian Rhythm Signaling Peptides and ProteinsPhosphorylationBacterial ProteinsCircadian Rhythm Signaling Peptides and ProteinscircadiancyanobacteriaKaiCRbp2RNA-binding protein

Identifiers

PMID37515350
PMCPMC10528358
OpenAlexW4385377503

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.