Evidence map›Paper›PMID 37530556›Full record

ArticlemBio2023

A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis.

Amanda T Alker, Morgan V Farrell, Alpher E Aspiras, Tiffany L Dunbar, Andriy Fedoriouk, Jeffrey E Jones, Sama R Mikhail, Gabriella Y Salcedo, Bradley S Moore, Nicholas J Shikuma

Abstract read
In one paragraph

Article in mBio, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Development of a CRISPR/Cas9-induced gene editing system forApplied and environmental microbiology · 2025
    Article
  3. Review
  4. MyD88 knockdown by RNAi prevents bacterial stimulation of tubeworm metamorphosis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. A modular toolkit for environmentalApplied and environmental microbiology · 2024
    Article
  10. Review
  11. Future research directions of the model marine tubeworm Hydroides elegans and synthesis of developmental staging of the complete life cycle.Developmental dynamics : an official publication of the American Association of Anatomists · 2023
    Article
  12. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Amanda T AlkerDepartment of Biology, San Diego State University , San Diego, California, USA.ORCID 0000-0002-4428-0978
Morgan V FarrellDepartment of Biology, San Diego State University , San Diego, California, USA.ORCID 0009-0007-7765-1521
Alpher E AspirasDepartment of Biology, San Diego State University , San Diego, California, USA.
Tiffany L DunbarDepartment of Biology, San Diego State University , San Diego, California, USA.
Andriy FedorioukDepartment of Biology, San Diego State University , San Diego, California, USA.
Jeffrey E JonesDepartment of Biology, San Diego State University , San Diego, California, USA.
Sama R MikhailDepartment of Biology, San Diego State University , San Diego, California, USA.
Gabriella Y SalcedoDepartment of Biology, San Diego State University , San Diego, California, USA.
Bradley S MooreCenter for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California , San Diego, California, USA.ORCID 0000-0002-4652-1253
Nicholas J ShikumaDepartment of Biology, San Diego State University , San Diego, California, USA.ORCID 0000-0001-5518-5020

Funding

Examining a Unique Contractile Injection System Mediating Host-Microbe InteractionsR35GM146722 · NIGMS · SAN DIEGO STATE UNIVERSITY · PI Nicholas J Shikuma · 2022 to 2026
$1.9M
Natural Sources and Microbial Transformation of Marine Halogenated PollutantsR01ES030316 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ALLEN, ERIC ELLSWORTH, MOORE, BRADLEY S · 2018 to 2022
$669k
NIEHS NIH HHS R01 ES030316NIGMS NIH HHS R35 GM146722
6 · The paper itself

Abstract

A conspicuous roadblock to studying marine bacteria for fundamental research and biotechnology is a lack of modular synthetic biology tools for their genetic manipulation. Here, we applied, and generated new parts for, a modular plasmid toolkit to study marine bacteria in the context of symbioses and host-microbe interactions. To demonstrate the utility of this plasmid system, we genetically manipulated the marine bacterium

Indexed as

BiotechnologyGenetic EngineeringAnimalsGenetic TechniquesPlasmidsProteobacteriaCRISPRigolden gateHydroidesmarinemetamorphosismodularsymbiosistubewormviolacein

Identifiers

PMID37530556
PMCPMC10470607

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.