Evidence mapPaperPMID 37537367Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2023

ACE2, ACE, DPPIV, PREP and CAT L enzymatic activities in COVID-19: imbalance of ACE2/ACE ratio and potential RAAS dysregulation in severe cases.

Raquel Leão Neves, Jéssica Branquinho, Júlia Galanakis Arata, Clarissa Azevedo Bittencourt, Caio Perez Gomes, Michelle Riguetti, Gustavo Ferreira da Mata, Danilo Euclides Fernandes, Marcelo Yudi Icimoto, Gianna Mastroianni Kirsztajn and 1 more

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In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. ACE2: the node connecting the lung cancer and COVID-19.American journal of cancer research · 2024
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Raquel Leão Neves *Department of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil.
Jéssica Branquinho *Department of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil.
Júlia Galanakis ArataDepartment of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil.
Clarissa Azevedo BittencourtDepartment of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil.
Caio Perez GomesDepartment of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil.
Michelle RiguettiDivision of Nephrology, Department of Medicine, Federal University of São Paulo, São Paulo, Brazil.
Gustavo Ferreira da MataDivision of Nephrology, Department of Medicine, Federal University of São Paulo, São Paulo, Brazil.
Danilo Euclides FernandesDivision of Nephrology, Department of Medicine, Federal University of São Paulo, São Paulo, Brazil.
Marcelo Yudi IcimotoDepartment of Biophysics, Federal University of São Paulo, São Paulo, Brazil. icimoto@unifesp.br.
Gianna Mastroianni KirsztajnDivision of Nephrology, Department of Medicine, Federal University of São Paulo, São Paulo, Brazil. gm.kirsztajn@unifesp.br.
João Bosco PesqueroDepartment of Biophysics, Center for Research and Molecular Diagnostic of Genetic Diseases, Federal University of São Paulo, São Paulo, Brazil. jbpesquero@unifesp.br.
Universidade Federal de São Paulo · BR

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 423165/2021-6Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/27198-8Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/01487-7Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/05266-5
6 · The paper itself

Abstract

OBJECTIVE AND

designCirculating enzymatic activity and RAAS regulation in severe cases of COVID-19 remains unclear, therefore we measured the serum activity of several proteases as potential targets to control the SARS-CoV-2 infection. MATERIAL OR SUBJECTS: 152 patients with COVID-19-like symptoms were grouped according to the severity of symptoms (COVID-19 negative, mild, moderate and severe).

methodsSerum samples of COVID-19 patients and controls were subjected to biochemical analysis and enzymatic assays of ACE2, ACE, DPPIV, PREP and CAT L. One-way ANOVA and multivariate logistic regression analysis were used. Statistical significance was accepted at p < 0.05.

resultsWe detected a positive correlation among comorbidities, higher C-reactive protein (CRP) and D-dimer levels with disease severity. Enzymatic assays revealed an increase in serum ACE2 and CAT L activities in severe COVID-19 patients, while ACE, DPPIV and PREP activities were significantly reduced. Notably, analysis of ACE2/ACE activity ratio suggests a possible imbalance of ANG II/ANG(1-7) ratio, in a positive association with the disease severity.

conclusionOur findings reveal a correlation between proteases activity and the severity of COVID-19. These enzymes together contribute to the activation of pro-inflammatory pathways, trigger a systemic activation of inflammatory mediators, leading to a RAAS dysregulation and generating a significant damage in several organs, contributing to poor outcomes of severe cases.

Indexed as

COVID-19Angiotensin-Converting Enzyme 2HumansPeptidyl-Dipeptidase ARenin-Angiotensin SystemAngiotensin-Converting Enzyme 2Peptidyl-Dipeptidase AACE2COVID-19ProteasesRAASSARS-CoV-2

Identifiers

PMID37537367
OpenAlexW4385516133

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.