Evidence map›Paper›PMID 37548922›Full record

ArticleAngewandte Chemie (International ed. in English)2023

A Semisynthetic Bioluminescence Sensor for Ratiometric Imaging of Metal Ions In Vivo Using DNAzymes Conjugated to An Engineered Nano-Luciferase.

Mengyi Xiong, Yuting Wu, Gezhi Kong, Whitney Lewis, Zhenglin Yang, Hanxiao Zhang, Li Xu, Ying Liu, Qin Liu, Xuhua Zhao and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Bioluminescent Systems for Theranostic Applications.International journal of molecular sciences · 2024
    Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Mengyi XiongMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.ORCID 0000-0001-9826-2284
Yuting WuDepartment of Chemistry, University of Texas at Austin, 78712, Austin, TX, USA.ORCID 0000-0003-3196-5916
Gezhi KongMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.
Whitney LewisDepartment of Chemistry, University of Texas at Austin, 78712, Austin, TX, USA.ORCID 0000-0003-1203-6441
Zhenglin YangDepartment of Chemistry, University of Texas at Austin, 78712, Austin, TX, USA.ORCID 0000-0003-2743-5779
Hanxiao ZhangDepartment of Biochemistry and Molecular Biology, Shanxi Medical University, 030001, Taiyuan, Shanxi, P. R. China.
Li XuMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.
Ying LiuMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.
Qin LiuMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.
Xuhua ZhaoDepartment of Biochemistry and Molecular Biology, Shanxi Medical University, 030001, Taiyuan, Shanxi, P. R. China.
Xiao-Bing ZhangMolecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, 410082, Changsha, Hunan, P. R. China.
Yi LuDepartment of Chemistry, University of Texas at Austin, 78712, Austin, TX, USA.ORCID 0000-0003-1221-6709
Hunan University · CNThe University of Texas at Austin · USShanxi Medical University · CN

Funding

Design and Selection of Novel Metalloenzymes for Biocatalysis, Bioimaging, and Genetic EngineeringR35GM141931 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Yi Lu · 2021 to 2026
$3.5M
NIGMS NIH HHS R35 GM141931
6 · The paper itself

Abstract

DNA-based probes have gained significant attention as versatile tools for biochemical analysis, benefiting from their programmability and biocompatibility. However, most existing DNA-based probes rely on fluorescence as the signal output, which can be problematic due to issues like autofluorescence and scattering when applied in complex biological materials such as living cells or tissues. Herein, we report the development of bioluminescent nucleic acid (bioLUNA) sensors that offer laser excitation-independent and ratiometric imaging of the target in vivo. The system is based on computational modelling and mutagenesis investigations of a genetic fusion between circular permutated Nano-luciferase (NLuc) and HaloTag, enabling the conjugation of the protein with a DNAzyme. In the presence of Zn

Indexed as

DNA, CatalyticFluorescence Resonance Energy TransferHumansIonsLuciferasesMaleMetalsDNA, CatalyticIonsLuciferasesMetalsBioluminescenceDNAzymeIn Vivo ImagingNucleic Acid SensorProtein Engineering

Identifiers

PMID37548922
PMCPMC10527972
OpenAlexW4385619802

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.