Evidence map›Paper›PMID 37558951›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

3D Spheroid Invasion Assay for High-Throughput Screening of Small-Molecule Libraries.

Kunal Karve, Stephanie Poon, Panagiotis Prinos, Laurie Ailles

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 4 institutions in 1 country.

Kunal KarvePrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Stephanie PoonPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada.
Panagiotis PrinosStructural Genomics Consortium, University of Toronto, Toronto, ON, Canada.
Laurie AillesPrincess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada. laurie.ailles@uhnresearch.ca.
Princess Margaret Cancer Centre · CAStructural Genomics Consortium · CAUniversity Health Network · CAUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer metastasis is a complex cascade that involves the activation of cancer cell migration and invasion of the extracellular space. Cancer-associated fibroblasts (CAFs) are known inducers of cancer cell invasion. However, current in vitro invasion assays such as the Boyden chamber assay are cumbersome and low throughput. Therefore, there is an urgent need for new ex vivo, surrogate invasion assays that can faithfully recapitulate the cancer cell invasion process in vitro and are amenable to large-scale screening of small-molecule libraries in a high-throughput fashion. Here, we describe a well-established high-throughput three-dimensional (3D) spheroid invasion assay as a powerful tool to identify novel molecular targets that can potentially mediate CAF-dependent cancer cell invasion.

Indexed as

High-Throughput Screening AssaysSmall Molecule LibrariesCell Line, TumorCell MovementHumansNeoplasm InvasivenessSpheroids, CellularSmall Molecule LibrariesCancer-associated fibroblastsCancer cellsHigh-throughputInvasionSmall-molecule librarySpheroidThree-dimensional (3D)

Identifiers

PMID37558951
OpenAlexW4385711956

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.