Evidence map›Paper›PMID 37572281›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Characterization of RNA Nanoparticles and Their Dynamic Properties Using Atomic Force Microscopy.

Alexander J Lushnikov, Yelixza I Avila, Kirill A Afonin, Alexey V Krasnoslobodtsev

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Alexander J LushnikovNanoimaging Core Facility at the University of Nebraska Medical Center, Omaha, NE, USA.
Yelixza I AvilaDepartment of Chemistry, Nanoscale Science Program, University of North Carolina, Charlotte, NC, USA.
Kirill A AfoninDepartment of Chemistry, Nanoscale Science Program, University of North Carolina, Charlotte, NC, USA.
Alexey V KrasnoslobodtsevDepartment of Physics, University of Nebraska Omaha, Omaha, NE, USA. akrasnos@unomaha.edu.

Funding

SMART NANPs: new molecular platform for communication with human immune system and modulation of therapeutic responsesR35GM139587 · NIGMS · UNIVERSITY OF NORTH CAROLINA CHARLOTTE · PI AFONIN, KIRILL A · 2021 to 2025
$1.8M
NIGMS NIH HHS R35 GM139587
6 · The paper itself

Abstract

The protocol described in this chapter allows for acquiring topography images of RNA-based nanoring structures and assessing their dynamic properties using atomic force microscopy (AFM) imaging. AFM is an indispensable tool for characterization of nucleic acid-based nanostructures with the exceptional capability of observing complexes in the range of a few nanometers. This method can visualize structural characteristics and evaluate differences between individual structurally different RNA nanorings. Due to the highly resolved AFM topography images, we introduce an approach that allows to distinguish the differences in the dynamic behavior of RNA nanoparticles not amenable to other experimental techniques. This protocol describes in detail the preparation procedures of RNA nanostructures, AFM imaging, and data analysis.

Indexed as

NanoparticlesNanostructuresMicroscopy, Atomic ForceRNARNAAtomic force microscopyFlexibility analysisMechanical stabilityMica surface modificationRNA nanoparticleTopography imaging

Identifiers

PMID37572281
PMCPMC10483931

What Socratic holds

Textmetadata
LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.