Evidence map›Paper›PMID 37573509›Full record

ArticleCell reports2023

TIGER: Single-step in vivo genome editing in a non-traditional rodent.

Sha Li, Sarah A Mereby, Megan Rothstein, Matthew R Johnson, Benjamin J Brack, Ricardo Mallarino

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Genome engineering with Cas9 and AAV repair templates, successes and pitfalls.Mammalian genome : official journal of the International Mammalian Genome Society · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sha LiDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Sarah A MerebyDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Megan RothsteinDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Matthew R JohnsonDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Benjamin J BrackDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA.
Ricardo MallarinoDepartment of Molecular Biology, Princeton University, Princeton, NJ 08540, USA. Electronic address: rmallarino@princeton.edu.

Funding

PREDOCTORAL TRAINING PROGRAM IN GENETICST32GM007388 · NIGMS · PRINCETON UNIVERSITY · PI CRISTEA, ILEANA M. · 1985 to 2022
$27.4M
Using naturally evolved phenotypic variation to decipher the positional regulatory code of mammalian skinR35GM133758 · NIGMS · PRINCETON UNIVERSITY · PI Ricardo Mallarino · 2019 to 2026
$3.0M
NIGMS NIH HHS R35 GM133758NIGMS NIH HHS T32 GM007388
6 · The paper itself

Abstract

Rodents are taxonomically diverse and have evolved a variety of traits. A mechanistic understanding of such traits has remained elusive, however, largely because genome editing in non-traditional model species remains challenging. Here, using the African striped mouse (Rhabdomys pumilio), we describe TIGER (targeted in vivo genome editing in rodents), a method that relies on a simple intraoviductal injecting technique and uses recombinant adeno-associated viruses (rAAVs) as the sole vehicle to deliver reagents into pregnant females. We demonstrate that TIGER generates knockout and knockin (up to 3 kb) lines with high efficiency. Moreover, we engineer a double-cleaving repair rAAV template and find that it significantly increases knockin frequency and germline transmission rates. Lastly, we show that an oversized double-cleaving rAAV template leads to an insertion of 3.8 kb. Thus, TIGER constitutes an attractive alternative to traditional ex vivo genome-editing methods and has the potential to be extended to a broad range of species.

Indexed as

Gene EditingAnimalsFemaleMicePregnancyRodentiaAfrican striped miceCP: Developmental biologyCP: Molecular biologyCRISPRCRISPR knock-inCRISPR knock-outemerging model organismsintraoviductal injectionsin vivo genome editingnon-traditional rodentsRhabdomys pumilio

Identifiers

PMID37573509
PMCPMC10528174

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.