Evidence map›Paper›PMID 37609337›Full record

ArticlebioRxiv : the preprint server for biology2023

Illuminating the Function of the Orphan Transporter, SLC22A10 in Humans and Other Primates.

Sook Wah Yee, Luis Ferrández-Peral, Pol Alentorn, Claudia Fontsere, Merve Ceylan, Megan L Koleske, Niklas Handin, Virginia M Artegoitia, Giovanni Lara, Huan-Chieh Chien and 10 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

20 authors at 8 institutions in 6 countries.

Sook Wah YeeDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Luis Ferrández-PeralInstitute of Evolutionary Biology (UPF-CSIC), PRBB, 08003 Barcelona, Spain.
Pol AlentornInstitute of Evolutionary Biology (UPF-CSIC), PRBB, 08003 Barcelona, Spain.
Claudia FontsereInstitute of Evolutionary Biology (UPF-CSIC), PRBB, 08003 Barcelona, Spain; Center for Evolutionary Hologenomics, The Globe Institute, University of Copenhagen, Øster Farimagsgade 5A, 1352 Copenhagen, Denmark.
Merve CeylanDepartment of Pharmacy and Science for Life Laboratory, Uppsala University, P.O. Box 580, 75123, Uppsala, Sweden.
Megan L KoleskeDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Niklas HandinDepartment of Pharmacy and Science for Life Laboratory, Uppsala University, P.O. Box 580, 75123, Uppsala, Sweden.
Virginia M ArtegoitiaUnited States Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, Davis, CA 95616, USA.
Giovanni LaraDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Huan-Chieh ChienDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Xujia ZhouDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Jacques DainatJoint Research Unit for Infectious Diseases and Vectors Ecology Genetics Evolution and Control (MIVEGEC), University of Montpellier, French National Center for Scientific Research (CNRS 5290), French National Research Institute for Sustainable Development (IRD 224), 911 Avenue Agropolis, BP 64501, 34394 Montpellier Cedex 5, France.
Arthur ZalevskyDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
Andrej SaliDepartment of Bioengineering and Therapeutic Sciences, UCSF Box 0775 1700 4th St, University of California, San Francisco, San Francisco, CA 94158, United States; Department of Pharmaceutical Chemistry, University of California, San Francisco, UCSF Box 2880 600 16th St, San Francisco, CA 94143, United States; Quantitative Biosciences Institute (QBI), University of California, San Francisco, 1700 4th St, San Francisco, CA, United States.
Colin M BrandBakar Computational Health Sciences Institute, University of California, San Francisco, CA, USA; Department of Epidemiology and Biostatistics, University of California, San Francisco, CA, USA.
John A CapraBakar Computational Health Sciences Institute, University of California, San Francisco, CA, USA; Department of Epidemiology and Biostatistics, University of California, San Francisco, CA, USA.
Per ArturssonDepartment of Pharmacy and Science for Life Laboratory, Uppsala University, P.O. Box 580, 75123, Uppsala, Sweden.
John W NewmanUnited States Department of Agriculture, Agricultural Research Service, Western Human Nutrition Research Center, Davis, CA 95616, USA; Department of Nutrition, University of California, Davis, Davis, CA 95616, USA; UC Davis West Coast Metabolomics Center, Davis, CA 95616, USA.
Tomas Marques-BonetInstitute of Evolutionary Biology (UPF-CSIC), PRBB, 08003 Barcelona, Spain; Institute of Evolutionary Biology (UPF-CSIC), PRBB, Dr. Aiguader 88, 08003 Barcelona, Spain; Catalan Institution of Research and Advanced Studies (ICREA), Passeig de Lluís Companys, 23, 08010, Barcelona, Spain; CNAG, Centro Nacional de Analisis Genomico, Barcelona Institute of Science and Technology (BIST), Baldiri i Reixac 4, 08028 Barcelona, Spain; Institut Català de Paleontologia Miquel Crusafont, Universitat Autònoma de Barcelona, Edifici ICTA-ICP, c/ Columnes s/n, 08193 Cerdanyola del Vallès, Barcelona, Spain.
Kathleen M GiacominiDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, California, USA.
University of California, San Francisco · USUppsala University · SEAgricultural Research Service · USInstitut de Biologia Evolutiva · ESCentre National de la Recherche Scientifique · FRInstitució Catalana de Recerca i Estudis Avançats · ESQB3 · USUniversity of Copenhagen · DK

Funding

Equipment Supplement for Discovery of Pharmacogenomic Biomarkers for OATP1B1 and OATP1B3R01GM117163 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI FLOREZ, JOSE CARLOS, HEDDERSON, MONIQUE MARIE · 2015 to 2023
$5.5M
The Evolution of Gene Regulation and Human DiseaseR35GM127087 · NIGMS · VANDERBILT UNIVERSITY · PI John Anthony Capra · 2018 to 2026
$3.2M
Equipment Supplement NOT-GM-24-021R01GM139875 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GIACOMINI, KATHLEEN M · 2021 to 2024
$1.9M
NIGMS NIH HHS R01 GM117163NIGMS NIH HHS R01 GM139875NIGMS NIH HHS R35 GM127087
6 · The paper itself

Abstract

SLC22A10 is classified as an orphan transporter with unknown substrates and function. Here we describe the discovery of the substrate specificity and functional characteristics of SLC22A10. The human SLC22A10 tagged with green fluorescent protein was found to be absent from the plasma membrane, in contrast to the SLC22A10 orthologs found in great apes. Estradiol-17β-glucuronide accumulated in cells expressing great ape SLC22A10 orthologs (over 4-fold, p<0.001). In contrast, human SLC22A10 displayed no uptake function. Sequence alignments revealed two amino acid differences including a proline at position 220 of the human SLC22A10 and a leucine at the same position of great ape orthologs. Site-directed mutagenesis yielding the human SLC22A10-P220L produced a protein with excellent plasma membrane localization and associated uptake function. Neanderthal and Denisovan genomes show human-like sequences at proline 220 position, corroborating that SLC22A10 were rendered nonfunctional during hominin evolution after the divergence from the pan lineage (chimpanzees and bonobos). These findings demonstrate that human SLC22A10 is a unitary pseudogene and was inactivated by a missense mutation that is fixed in humans, whereas orthologs in great apes transport sex steroid conjugates.

Identifiers

PMID37609337
PMCPMC10441401
OpenAlexW4385778626

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.