Evidence map›Paper›PMID 37624482›Full record

ArticleMolecular biotechnology2024

Functional Mutations in the microRNA-155 Promoter Modulate its Transcription Efficiency and Expression.

Congcong Li, Wanxia Zhao, Huijie Zhou, Jiao Wu, Yong Huo, Dongfeng Jiang, Xiangbo Ji, Kun Liu, Qiuliang Xu, Wantao Li

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Congcong LiCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China. congcong_925520@126.com.ORCID http://orcid.org/0000-0002-0914-8955
Wanxia ZhaoCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Huijie ZhouCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Jiao WuCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Yong HuoCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Dongfeng JiangCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Xiangbo JiHenan Key Laboratory of Unconventional Feed Resources Innovative Utilization, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Kun LiuHenan Key Laboratory of Unconventional Feed Resources Innovative Utilization, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
Qiuliang XuCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.
Wantao LiCollege of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China.

Funding

National Natural Science Foundation of China 31702102
6 · The paper itself

Abstract

Limited research has been conducted on porcine miR-155 promoters, and previous study from our group have identified two haplotypes (TT and CC) in different pig breeds, each associated with five fully linked mutation sites within or near the miR-155 gene (Li et al. Dev Comp Immunol 39(1):110-116, 2013). In this study, the promoter region of porcine miR-155 was screened, and two important transcription factors, Foxp3 and RelA, were identified. The binding ability of Foxp3 protein was found to be affected by the first mutation site (A/C) using EMSA analysis. In vitro experiments revealed that the expression level of miR-155 was significantly higher in the C haplotype compared to the T haplotype. Additionally, northern blotting assays indicated that both the first mutation site (A/C) and the fourth mutation site (G/T) had a significant impact on miR-155 expression levels. These findings provide further insights into the transcriptional regulation of porcine miR-155 and identify crucial mutation sites that influence miR-155 expression. This knowledge can serve as a basis for identifying potential molecular markers associated with disease resistance in swine.

Indexed as

MicroRNAsMutationPromoter Regions, GeneticAnimalsForkhead Transcription FactorsGene Expression RegulationHaplotypesSwineTranscription, GeneticForkhead Transcription FactorsMicroRNAsFunctional mutationsPorcine miR-155The expression levelTranscriptional regulation

Identifiers

PMID37624482

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.