Evidence map›Paper›PMID 37626628›Full record

ArticleBiomedicines2023

Myocardial RNA Sequencing Reveals New Potential Therapeutic Targets in Heart Failure with Preserved Ejection Fraction.

José M Inácio, Fernando Cristo, Miguel Pinheiro, Francisco Vasques-Nóvoa, Francisca Saraiva, Mafalda M Nunes, Graça Rosas, Andreia Reis, Rita Coimbra, José Luís Oliveira and 3 more

Open access · goldAbstract read
In one paragraph

Article in Biomedicines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Epigenetic Mechanisms in Heart Diseases.Reviews in cardiovascular medicine · 2025
    Review
  6. Article
  7. miRNAs in Heart Development and Disease.International journal of molecular sciences · 2024
    Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

José M InácioStem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.ORCID 0000-0002-9790-8335
Fernando CristoStem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.
Miguel PinheiroGenome Medicine Lab, Department of Medical Sciences, Institute for Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.ORCID 0000-0002-6601-7513
Francisco Vasques-NóvoaCardiovascular R&D Centre-UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine, University of Porto, 1169-056 Porto, Portugal.
Francisca SaraivaCardiovascular R&D Centre-UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine, University of Porto, 1169-056 Porto, Portugal.
Mafalda M NunesStem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.ORCID 0000-0001-8961-3097
Graça RosasStem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.
Andreia ReisGenome Medicine Lab, Department of Medical Sciences, Institute for Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Rita CoimbraGenome Medicine Lab, Department of Medical Sciences, Institute for Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
José Luís OliveiraInstitute of Electronics and Informatics Engineering of Aveiro (IEETA), University of Aveiro, 3810-193 Aveiro, Portugal.ORCID 0000-0002-6672-6176
Gabriela MouraGenome Medicine Lab, Department of Medical Sciences, Institute for Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.ORCID 0000-0003-2243-6123
Adelino Leite-MoreiraCardiovascular R&D Centre-UnIC@RISE, Department of Surgery and Physiology, Faculty of Medicine, University of Porto, 1169-056 Porto, Portugal.ORCID 0000-0001-7808-3596
José António BeloStem Cells and Development Laboratory, iNOVA4Health, NOVA Medical School|Faculdade de Ciências Médicas, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal.ORCID 0000-0001-7384-0949
Universidade Nova de Lisboa · PTUniversity of Aveiro · PTUniversidade do Porto · PT

Funding

Fundação para a Ciência e Tecnologia iNOVA4Health-UID/Multi/04462/2020Fundação para a Ciência e Tecnologia NETDIAMOND-SAICTPAC/0047/2015Fundação para a Ciência e Tecnologia UID/BIM/04501/2020; POCI-01-0145-FEDER-022184; UIDB/00051/2020; UIDP/00051/2020; LA/P/0053/2020
6 · The paper itself

Abstract

Heart failure with preserved ejection fraction (HFpEF) represents a global health challenge, with limited therapies proven to enhance patient outcomes. This makes the elucidation of disease mechanisms and the identification of novel potential therapeutic targets a priority. Here, we performed RNA sequencing on ventricular myocardial biopsies from patients with HFpEF, prospecting to discover distinctive transcriptomic signatures. A total of 306 differentially expressed mRNAs (DEG) and 152 differentially expressed microRNAs (DEM) were identified and enriched in several biological processes involved in HF. Moreover, by integrating mRNA and microRNA expression data, we identified five potentially novel miRNA-mRNA relationships in HFpEF: the upregulated hsa-miR-25-3p, hsa-miR-26a-5p, and has-miR4429, targeting HAPLN1; and NPPB mRNA, targeted by hsa-miR-26a-5p and miR-140-3p. Exploring the predicted miRNA-mRNA interactions experimentally, we demonstrated that overexpression of the distinct miRNAs leads to the downregulation of their target genes. Interestingly, we also observed that microRNA signatures display a higher discriminative power to distinguish HFpEF sub-groups over mRNA signatures. Our results offer new mechanistic clues, which can potentially translate into new HFpEF therapies.

Indexed as

heart failureHFpEFintercellular communicationmiRNA–mRNAmiRNA signature in HFpEF

Identifiers

PMID37626628
PMCPMC10452106
OpenAlexW4385341293

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.