Evidence map›Paper›PMID 37650322›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2023

Loss of 12-Lipoxygenase Improves the Post-Transfusion Function of Stored Platelets.

Hannah J Larsen, Daire Byrne, Tahsin Özpolat, Aastha Chauhan, S Lawrence Bailey, Nicole Rhoads, Franklin Reed, Massiel C Stolla, Reheman Adili, Michael Holinstat and 2 more

Erratum issuedOpen access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 12 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Hannah J Larsen *Bloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
Daire Byrne *Bloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
Tahsin ÖzpolatBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).ORCID 0000-0001-7527-3933
Aastha ChauhanBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
S Lawrence BaileyBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
Nicole RhoadsBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).ORCID 0000-0001-8331-5136
Franklin ReedBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
Massiel C StollaDivision of Hematology, Department of Medicine (M.C.S., X.F., M.S.), University of Washington Medical Center, Seattle.ORCID 0000-0003-0432-6617
Reheman AdiliBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).ORCID 0000-0002-1973-9695
Michael HolinstatDepartment of Pharmacology, University of Michigan, Ann Arbor (M.H.).ORCID 0000-0001-5100-1933
Xiaoyun FuBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).
Moritz StollaBloodworks Northwest Research Institute, Seattle, WA (H.J.L., D.B., T.Ö., A.C., S.L.B., N.R., F.R., R.A., X.F., M.S.).ORCID 0000-0002-2866-1674
Bloodworks Northwest · USUniversity of Michigan · US

Funding

Role of TRPM8 in Cold-stored Platelet TransfusionsR01HL153072 · NHLBI · BLOODWORKS · PI STOLLA, MORITZ · 2020 to 2024
$2.6M
12-HETrE regulation of blood coagulation, hemostasis, and thrombosisR35GM131835 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HOLINSTAT, MICHAEL ALLAN · 2019 to 2023
$2.3M
NHLBI NIH HHS R01 HL153072NIGMS NIH HHS R35 GM131835
6 · The paper itself

Abstract

backgroundPlatelets for transfusion are stored for 5 to 7 days. Previous studies have shown that HETE levels in the storage bag negatively correlate with platelet performance in vivo, suggesting that the dysregulation of bioactive lipid mediators may contribute to the storage lesion. In the current study, we sought to understand how genetic deletion and pharmacological inhibition of 12-LOX (12-lipoxygenase) affects platelets during storage and after transfusion.

methodsPlatelets from 12-LOX

resultsPolyunsaturated fatty acids increased significantly in stored platelets from 12-LOX

conclusionsDeleting 12-LOX improves the post-transfusion function of stored murine platelets by increasing thromboxane generation through COX-1-dependent arachidonic acid metabolism. Future studies should determine the feasibility and safety of 12-LOX-inhibited platelets transfused to humans.

Indexed as

Arachidonate 12-LipoxygenaseBlood PlateletsAnimalsHumansMiceMice, Inbred NODMice, SCIDMice, TransgenicPlatelet Glycoprotein GPIIb-IIIa ComplexThromboxanesArachidonate 12-LipoxygenasePlatelet Glycoprotein GPIIb-IIIa ComplexThromboxanesarachidonic acidblood plateletshemostasislipoxygenasesmice

Identifiers

PMID37650322
PMCPMC10538391
OpenAlexW4386305461

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.