Evidence map›Paper›PMID 37659708›Full record

ArticleExperimental eye research2023

A simple, rapid fluorescent reporter-based method for detection of ectopic cre recombinase expression in presumed retinal cell type-targeted mouse lines.

Sriganesh Ramachandra Rao, Steven J Fliesler

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental eye research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Sriganesh Ramachandra RaoDepartment of Ophthalmology, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Neuroscience Graduate Program, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Research Service, VA Western New York Healthcare System, Buffalo, NY, USA.
Steven J FlieslerDepartment of Ophthalmology, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Neuroscience Graduate Program, Jacobs School of Medicine and Biomedical Sciences, The State University of New York - University at Buffalo, Buffalo, NY, USA; Research Service, VA Western New York Healthcare System, Buffalo, NY, USA. Electronic address: fliesler@buffalo.edu.
New York University · US

Funding

University of Buffalo Clinical and Translational Science Institute - Supplement SchulyerUL1TR001412 · NCATS · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI MURPHY, TIMOTHY F · 2015 to 2024
$33.8M
ISOPRENOID METABOLISM IN THE RETINAR01EY007361 · NEI · UNIVERSITY OF SOUTH FLORIDA · PI FLIESLER, STEVEN J. · 1988 to 2015
$4.4M
Development and characterization of mouse models of RP59 DHDDS deficiencyR01EY029341 · NEI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI FLIESLER, STEVEN J., PITTLER, STEVEN J · 2018 to 2021
$2.2M
Cholesterol homeostasis in the vertebrate retinaR01EY033298 · NEI · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI Steven J. Fliesler · 2023 to 2026
$1.5M
BLRD Research Career Scientist Award ApplicationIK6BX005787 · VA · VA WESTERN NEW YORK HEALTHCARE SYSTEM · PI Steven J. Fliesler · 2022 to 2026
–
BLRD VA IK6 BX005787NCATS NIH HHS UL1 TR001412NEI NIH HHS R01 EY007361NEI NIH HHS R01 EY029341NEI NIH HHS R01 EY033298
6 · The paper itself

Abstract

Although cell type-specific Cre recombinase-expressing mouse lines are commonly used to generate conditional knockout of genes of interest, germline recombination and ectopic "leakiness" in Cre recombinase expression in non-specific cell types has been observed in several neuronal and glial-specific Cre lines. This often leads to inadvertent loss of conditional mouse lines, requiring rederivation. It is therefore imperative to be able to monitor and validate cell type-specific Cre recombinase-mediated gene editing. Herein, we describe a simple, inexpensive, rapid ZsGreen fluor-reporter-based strategy for genotype-free identification of ectopic leakiness using a custom-designed, 3-D blue LED light box. We assessed cell type-specific expression in several allegedly specific Cre recombinase mouse lines commonly used in vision research: retinal pigment epithelium (RPE)-specific (VMD2 (Best1) Cre, RPE65 Cre); astrocyte-specific (GFAP Cre); as well as photoreceptor-bipolar progenitor cell-specific (CRX Cre). Our standardized workflow allows facile, rapid identification of ectopic and non-specific Cre recombinase expression in any presume specific Cre mouse line, without the need for genotyping and without causing animal distress.

Indexed as

Coloring AgentsNeuronsAnimalsIntegrasesMiceStaining and LabelingColoring AgentsCre recombinaseIntegrasesConditional gene knockoutCre-loxCre recombinaseFluorescent reporterGermline recombination

Identifiers

PMID37659708
PMCPMC10756212
OpenAlexW4386319752

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.