Evidence map›Paper›PMID 37662299›Full record

ArticlebioRxiv : the preprint server for biology2023

ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements.

Brandon A Boone, Lucia Ichino, Shuya Wang, Jason Gardiner, Jaewon Yun, Yasaman Jami-Alahmadi, Jihui Sha, Cristy P Mendoza, Bailey J Steelman, Aliya van Aardenne and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Brandon A BooneMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Lucia IchinoMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Shuya WangMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Jason GardinerDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Jaewon YunDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Yasaman Jami-AlahmadiDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095, USA.
Jihui ShaDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095, USA.
Cristy P MendozaDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Bailey J SteelmanDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Aliya van AardenneDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Sophia Kira-LucasDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
Isabelle TrentchevDepartment of Molecular, Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA 90095, USA.
James A WohlschlegelDepartment of Biological Chemistry, University of California Los Angeles, Los Angeles, CA 90095, USA.
Steven E JacobsenMolecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.

Funding

Proteolytic Control of Iron Metabolism by the Ubiquitin Ligase FBXL5R01GM089778 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WOHLSCHLEGEL, JAMES AKIRA · 2010 to 2023
$4.0M
Epigenetic gene regulation in ArabidopsisR35GM130272 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI JACOBSEN, STEVEN E · 2019 to 2023
$1.6M
NIGMS NIH HHS R01 GM089778NIGMS NIH HHS R35 GM130272
6 · The paper itself

Abstract

DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG-binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline domain containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher order multimerization of MBD5/6 complexes within heterochromatin. These complexes are also highly dynamic, with the mobility of complex components regulated by the activity of SLN. Using a dCas9 system, we demonstrate that tethering the ACDs to an ectopic site outside of heterochromatin can drive massive accumulation of MBD5/6 complexes into large nuclear bodies. These results demonstrate that ACD15 and ACD21 are critical components of gene silencing complexes that act to drive the formation of higher order, dynamic assemblies.

Identifiers

PMID37662299
PMCPMC10473691

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.