Evidence map›Paper›PMID 37668922›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Scalable CRISPR Screens in Zebrafish Using MIC-Drop.

Saba Parvez, Tejia Zhang, Randall T Peterson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Saba ParvezDepartment of Pharmacology & Toxicology, University of Utah, Salt Lake City, UT, USA.
Tejia ZhangDepartment of Pharmacology & Toxicology, University of Utah, Salt Lake City, UT, USA.
Randall T PetersonDepartment of Pharmacology & Toxicology, University of Utah, Salt Lake City, UT, USA. randall.peterson@pharm.utah.edu.

Funding

In vivo functional screen of noncoding genetic elementsR00HG012593 · NHGRI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI Saba Parvez · 2024 to 2026
$747k
In vivo functional screen of noncoding genetic elementsK99HG012593 · NHGRI · UNIVERSITY OF UTAH · PI PARVEZ, SABA · 2022 to 2023
$142k
NHGRI NIH HHS K99 HG012593NHGRI NIH HHS R00 HG012593
6 · The paper itself

Abstract

CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging due to the cost, labor, and time required to generate and keep track of thousands of mutant animals. We reported the development of Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform that makes large-scale reverse genetic screens possible in zebrafish. In this chapter, we provide a detailed protocol to conduct large-scale genetic screens using this novel platform.

Indexed as

Cell Culture TechniquesZebrafishAnimalsTechnologyCRISPR-Cas9F0 CRISPR screenGenetic screenHigh-throughputIn vivoReverse geneticsZebrafish

Identifiers

PMID37668922

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.