Evidence map›Paper›PMID 37688729›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Preparation of Human Olfactory Epithelial Biopsies for Downstream Analysis.

Rhea Choi, Tiffany Ko, John B Finlay, Ralph Abi Hachem, David Jang, Bradley J Goldstein

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Rhea ChoiDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA.
Tiffany KoDepartment of Neurobiology, Duke University School of Medicine, Durham, NC, USA.
John B FinlayMedical Scientist Training Program, Duke University School of Medicine, Durham, NC, USA.
Ralph Abi HachemDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA.
David JangDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA.
Bradley J GoldsteinDepartment of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA. Bradley.goldstein@duke.edu.
Duke University · US

Funding

Therapeutic potential for modulation of olfactory basal stem cellsR01DC016859 · NIDCD · UNIVERSITY OF MIAMI SCHOOL OF MEDICINE · PI GOLDSTEIN, BRADLEY J · 2018 to 2022
$2.2M
Single cell analysis of human olfactory mucosaR21DC018371 · NIDCD · DUKE UNIVERSITY · PI GOLDSTEIN, BRADLEY J · 2020 to 2021
$443k
NIDCD NIH HHS R01 DC016859NIDCD NIH HHS R21 DC018371
6 · The paper itself

Abstract

The olfactory mucosa, lining a portion of the nasal cavity, houses the primary olfactory sensory neurons responsible for odor transduction, along with supporting cell populations. Tremendous advances have come from studying the peripheral olfactory system in animal models, especially the mouse. However, acquired human olfactory disorders lack effective therapies, and many of these conditions involve pathology in the olfactory mucosa. Thus, the ability to obtain human olfactory biopsy samples from subjects with olfactory dysfunction, or controls, may be of value. Here, we describe established techniques for collecting olfactory tissue from human subjects and preparing samples for downstream assays such as immunohistochemistry, flow cytometry, single-cell RNA-sequencing, or chromatin studies.

Indexed as

Biological AssaySmellAnimalsBiopsyChromatinFlow CytometryHumansMiceChromatinEndoscopic endonasal approachHuman olfactory epitheliumNasal cytologyOlfactory cleft

Identifiers

PMID37688729
OpenAlexW4386569164

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.