Evidence mapPaperPMID 37705447Full record

ArticleJournal of applied physiology (Bethesda, Md. : 1985)2023

Short-term repeated human biopsy sampling contributes to changes in muscle morphology and higher outcome variability.

Douglas E Long, Alessandra J Mantuano, Amy L Confides, Benjamin F Miller, Philip A Kern, Timothy A Butterfield, Esther E Dupont-Versteegden

Abstract read
In one paragraph

Article in Journal of applied physiology (Bethesda, Md. : 1985), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Douglas E LongDepartment of Physical Therapy, College of Health Sciences, University of Kentucky, Lexington, Kentucky, United States.ORCID 0000-0002-6770-5764
Alessandra J MantuanoCenter for Muscle Biology, University of Kentucky, Lexington, Kentucky, United States.
Amy L ConfidesDepartment of Physical Therapy, College of Health Sciences, University of Kentucky, Lexington, Kentucky, United States.
Benjamin F MillerAging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, United States.ORCID 0000-0003-3283-0685
Philip A KernDivision of Endocrinology, Department of Internal Medicine, Barnstable Brown Diabetes and Obesity Center, University of Kentucky, Lexington, Kentucky, United States.ORCID 0000-0002-4761-7417
Timothy A ButterfieldCenter for Muscle Biology, University of Kentucky, Lexington, Kentucky, United States.ORCID 0000-0002-8787-2148
Esther E Dupont-VersteegdenDepartment of Physical Therapy, College of Health Sciences, University of Kentucky, Lexington, Kentucky, United States.ORCID 0000-0001-5576-9659

Funding

Kentucky Center for Clinical and Translational ScienceUL1TR001998 · UNIVERSITY OF KENTUCKY · 2025 to 2025
$3.3M
NCATS NIH HHS UL1 TR001998NCCIH NIH HHS R21 AT010847
6 · The paper itself

Abstract

Changes in skeletal muscle are an important aspect of overall health. The collection of human muscle to study cellular and molecular processes for research requires a needle biopsy procedure which, in itself, can induce changes in the tissue. To investigate the effect of repeat tissue sampling, we collected skeletal muscle biopsy samples from vastus lateralis separated by 7 days. Cellular infiltrate, central nucleation, enlarged extracellular matrix, and rounding of muscle fibers were used as indices to define muscle damage, and we found that 16/26 samples (61.5%) revealed at least two of these symptoms in the secondary biopsy. The presence of damage influenced outcome measures usually obtained in human biopsies. Damaged muscle showed an increase in the number of small fibers even though average fiber and fiber type-specific cross-sectional area (CSA) were not different. This included higher numbers of embryonic myosin heavy chain-positive fibers (

Indexed as

Muscle, SkeletalSatellite Cells, Skeletal MuscleBiopsyHumansMuscle Fibers, SkeletalQuadriceps Musclebiopsy techniquehistologylocal anestheticmuscle damageskeletal muscle

Identifiers

PMID37705447
PMCPMC10979834

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.