Evidence map›Paper›PMID 37724007›Full record

ArticleVeterinary and comparative oncology2023

Sensitivity of osteosarcoma cell lines to autophagy inhibition as determined by pharmacologic and genetic manipulation.

Daniel L Gustafson, Lindsey O Viola, Christina G Towers, Sunetra Das, Dawn L Duval, Kristen M Van Eaton

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary and comparative oncology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Daniel L GustafsonSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado, USA.ORCID https://orcid.org/0000-0002-3063-1669
Lindsey O ViolaFlint Animal Cancer Center, Colorado State University, Fort Collins, Colorado, USA.
Christina G TowersMolecular and Cell Biology Laboratory, Salk Institute for Biological Sciences, La Jolla, California, USA.
Sunetra DasDepartment of Clinical Sciences, Colorado State University, Fort Collins, Colorado, USA.
Dawn L DuvalDepartment of Clinical Sciences, Colorado State University, Fort Collins, Colorado, USA.ORCID https://orcid.org/0000-0002-5310-0078
Kristen M Van EatonSchool of Biomedical Engineering, Colorado State University, Fort Collins, Colorado, USA.
Colorado State University · USUniversity of Colorado Cancer Center · USSalk Institute for Biological Studies · US

Funding

University of Colorado Cancer Center Support Grant - Lung Cancer Patient-Derived Xenografts with Autologous Human Immune SystemsP30CA046934 · NCI · UNIVERSITY OF COLORADO DENVER · PI James V Degregori · 1988 to 2026
$117.0M
Therapeutic targeting of autophagy-dependent cancerR01CA190170 · NCI · UNIVERSITY OF COLORADO DENVER · PI GUSTAFSON, DANIEL L, THORBURN, ANDREW M · 2015 to 2019
$2.5M
Therapeutic Targeting of Autophagy-Dependent CancerR00CA245187 · NCI · SALK INSTITUTE FOR BIOLOGICAL STUDIES · PI TOWERS, CHRISTINA G · 2021 to 2023
$816k
NCI NIH HHS P30 CA046934NCI NIH HHS R00 CA245187NCI NIH HHS R00CA245187NCI NIH HHS R01 CA190170NCI NIH HHS R01CA190170
6 · The paper itself

Abstract

Pharmacologic inhibition of autophagy can be achieved using lysosomotropic agents such as hydroxychloroquine (HCQ) that interfere with fusion of the autophagosome to the lysosome thus preventing completion of the recycling process. The goal of the present study is to determine the sensitivity of eight canine (cOSA) and four human (hOSA) osteosarcoma tumour cell lines to antiproliferative and cytotoxic effects of lysosomal autophagy inhibitors, and to compare these results to the autophagy-dependence measured using a CRISPR/Cas9 live-cell imaging assay in OSA and other tumour cell lines. Antiproliferative and cytotoxic response to HCQ and Lys05 was determined using live cell imaging and YOYO-1 staining. CRISPR/Cas9 live cell imaging screen was done using species specific guide RNA's and transfection of reagents into cells. Response to autophagy core genes was compared to response to an essential (PCNA) and non-essential (FOXO3A) gene. cOSA and hOSA cell lines showed similar antiproliferative and cytotoxic responses to HCQ and Lys05 with median lethal dose (D

Indexed as

Antineoplastic AgentsDog DiseasesOsteosarcomaAminoquinolinesAnimalsAutophagyCell Line, TumorDogsHumansHydroxychloroquinePolyaminesRNA, Guide, CRISPR-Cas SystemsAminoquinolinesAntineoplastic AgentsHydroxychloroquineLys01PolyaminesRNA, Guide, CRISPR-Cas Systemsautophagycomparative oncologyhydroxychloroquineLys05osteosarcoma

Identifiers

PMID37724007
PMCPMC11470750
OpenAlexW4386843207

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.