Evidence map›Paper›PMID 37734382›Full record

ArticleCell reports2023

ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization.

Hideki Nakamura, Elmer Rho, Christopher T Lee, Kie Itoh, Daqi Deng, Satoshi Watanabe, Shiva Razavi, Hideaki T Matsubayashi, Cuncheng Zhu, Eleanor Jung and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Chemical reviews · 2026
    Review
  3. Review
  4. Article
  5. Rac1 promotes proximal tubule kidney repair by coupling the actin cytoskeleton to mitochondrial function.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  6. Article
  7. Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Hideki NakamuraDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Kyoto University Hakubi Center for Advanced Research, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8501, Japan; Kyoto University Graduate School of Engineering, Department of Synthetic Chemistry and Biological Chemistry, Katsura Int'tech Center, Graduate School of Engineering, Kyoto University, Nishikyo-ku, Kyoto 615-8530, Japan. Electronic address: nakamura@sbchem.kyoto-u.ac.jp.
Elmer RhoDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Christopher T LeeDepartment of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093, USA.
Kie ItohDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Daqi DengDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Satoshi WatanabeDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Shiva RazaviDepartment of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Hideaki T MatsubayashiDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Cuncheng ZhuDepartment of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093, USA.
Eleanor JungDepartment of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093, USA.
Padmini RangamaniDepartment of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA 92093, USA.
Shigeki WatanabeDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Takanari InoueDepartment of Cell Biology and Center for Cell Dynamics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: jctinoue@jhmi.edu.
Johns Hopkins University · USUniversity of California San Diego · US

Funding

Decoding dynamic interplay between signaling and membranes in chemotaxis bymolecular actuatorsR35GM149329 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Takanari Inoue · 2023 to 2026
$2.8M
Reviving electron microscopy for synaptic cell biologyDP2NS111133 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2018
$2.5M
Spatial and molecular determinants of fusion probability and timingR01NS105810 · NINDS · JOHNS HOPKINS UNIVERSITY · PI WATANABE, SHIGEKI · 2018 to 2022
$1.8M
Feedback and Crosstalk in Eukaryotic ChemotaxisR01GM123130 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI INOUE, TAKANARI · 2018 to 2021
$1.5M
ActuAtor, a molecular tool for generating force in living cellsR01GM136858 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI INOUE, TAKANARI · 2020 to 2022
$983k
NIGMS NIH HHS R01 GM123130NIGMS NIH HHS R01 GM136858NIGMS NIH HHS R35 GM149329NINDS NIH HHS DP2 NS111133NINDS NIH HHS R01 NS105810
6 · The paper itself

Abstract

Form and function are often interdependent throughout biology. Inside cells, mitochondria have particularly attracted attention since both their morphology and functionality are altered under pathophysiological conditions. However, directly assessing their causal relationship has been beyond reach due to the limitations of manipulating mitochondrial morphology in a physiologically relevant manner. By engineering a bacterial actin regulator, ActA, we developed tools termed "ActuAtor" that inducibly trigger actin polymerization at arbitrary subcellular locations. The ActuAtor-mediated actin polymerization drives striking deformation and/or movement of target organelles, including mitochondria, Golgi apparatus, and nucleus. Notably, ActuAtor operation also disperses non-membrane-bound entities such as stress granules. We then implemented ActuAtor in functional assays, uncovering the physically fragmented mitochondria being slightly more susceptible to degradation, while none of the organelle functions tested are morphology dependent. The modular and genetically encoded features of ActuAtor should enable its application in studies of the form-function interplay in various intracellular contexts.

Indexed as

ListeriaListeria monocytogenesActinsBacterial ProteinsOrganellesPolymerizationActinsBacterial Proteinsactin polymerizationCP: Cell biologyform-function interplaymitochondriamolecular actuatorsphysical manipulationstress granules

Identifiers

PMID37734382
PMCPMC10872831
OpenAlexW4386898339

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.