Evidence mapPaperPMID 37736778Full record

ArticleBiotechnology letters2023

Magnetic particle-based chemiluminescence immunoassay for serum human heart-type fatty acid binding protein measurement.

Jiao Zheng, Yilan Qiu, Ye Xu, Meifang Quan, Zhihong Zhong, Qinglin Wang, Yi Wu, Xuan Zeng, Chuan Xia, Rushi Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jiao ZhengSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Yilan Qiu *Immunodiagnostic Reagents Engineering Research Center of Hunan Province, Hunan Normal University, Changsha, 410013, China.
Ye XuSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Meifang QuanSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Zhihong ZhongSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Qinglin WangSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Yi WuImmunodiagnostic Reagents Engineering Research Center of Hunan Province, Hunan Normal University, Changsha, 410013, China.
Xuan ZengSchool of Medicine, Hunan Normal University, Changsha, 410013, China.
Chuan XiaDepartment of Laboratory Medicine, The First People's Hospital of Chenzhou, Chenzhou, 423000, China.
Rushi LiuSchool of Medicine, Hunan Normal University, Changsha, 410013, China. liurushi@hunnu.edu.cn.ORCID http://orcid.org/0000-0002-1906-2514
Hunan Normal University · CNChenzhou First People's Hospital · CN

Funding

National Natural Science Foundation of China 81702005Natural Science Foundation of Hunan Province 2019JJ50370Science and Technology Plan of Hunan Provincial People's Hospital RS20160208Science and Technology Plan Project of Changsha KQ1801039Science and Technology Plan Project of Changsha KQ1907130Scientific Research Project of Hunan Provincial Health Commission B202311009257
6 · The paper itself

Abstract

objectivesHuman heart-type fatty acid binding protein (HFABP) is a biomarker for diagnosis, risk assessment, and prognosis of acute myocardial infarction, and we aimed to establish an immunoassay for HFABP quantitation.

methodsHuman HFABP monoclonal antibodies (mAbs) were developed, evaluated by enzyme-linked immunosorbent assay, and a chemiluminescence enzyme immunoassay (CLEIA) generated. Analytical performance of the CLEIA was evaluated by measuring serum HFABP.

resultsThe prokaryotically expressed rHFABP was purified and four anti-HFABP mAbs with superior detection performance were obtained after immunizing BALB/c mice. MAbs 2B8 and 6B3 were selected as respective capture and detection antibodies for HFABP measurement by CLEIA (detection range, 0.01-128 μg/L). Results using the CLEIA showed excellent correlation (r, 0.9622) and the correlation coefficient was 0.9809 (P < 0.05) by the Tukey test statistical analysis with those of latex-enhanced immunoturbidimetry in hospitals.

conclusionOur mAbs and CLEIA for HFABP detection represent new diagnostic tools for measurement of human serum HFABP.

Indexed as

Antibodies, MonoclonalLuminescenceAnimalsBiomarkersEnzyme-Linked Immunosorbent AssayHumansImmunoassayMiceAntibodies, MonoclonalBiomarkersChemiluminescence enzyme immunoassayDouble sandwich enzyme-linked immunosorbent assayHeart-type fatty acid binding proteinMonoclonal antibody

Identifiers

PMID37736778
OpenAlexW4386944213

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.