Evidence map›Paper›PMID 37745376›Full record

ArticlebioRxiv : the preprint server for biology2023

Integration of Patient-Derived Organoids and Organ-on-Chip Systems: Investigating Colorectal Cancer Invasion within the Mechanical and GABAergic Tumor Microenvironment.

Carly Strelez, Rachel Perez, John S Chlystek, Christopher Cherry, Ah Young Yoon, Bethany Haliday, Curran Shah, Kimya Ghaffarian, Ren X Sun, Hannah Jiang and 5 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Carly StrelezLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Rachel PerezLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
John S ChlystekLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Christopher CherryC M Cherry Consulting, Baltimore, MD, USA.
Ah Young YoonLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Bethany HalidayLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Curran ShahLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Kimya GhaffarianLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Ren X SunLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Hannah JiangLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Roy LauLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Aaron SchatzLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Heinz-Josef LenzDivision of Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Jonathan E KatzLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Shannon M MumenthalerLawrence J. Ellison Institute for Transformative Medicine, Los Angeles, CA, USA.
Larry Ellison Foundation · USUniversity of Southern California · USRED Consulting (Norway) · NO

Funding

USC/NORRIS COMPREHENSIVE CANCER CENTER (CORE) SUPPORTP30CA014089 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Fumito Ito · 1985 to 2026
$181.4M
A microengineered colon cancer-chip designed to investigate tumor-stromal interactions driving cancer progressionR01CA241137 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI MUMENTHALER, SHANNON MICHELLE · 2021 to 2025
$2.3M
NCI NIH HHS P30 CA014089NCI NIH HHS R01 CA241137
6 · The paper itself

Abstract

Three-dimensional (3D) in vitro models are essential in cancer research, but they often neglect physical forces. In our study, we combined patient-derived tumor organoids with a microfluidic organ-on-chip system to investigate colorectal cancer (CRC) invasion in the tumor microenvironment (TME). This allowed us to create patient-specific tumor models and assess the impact of physical forces on cancer biology. Our findings showed that the organoid-on-chip models more closely resembled patient tumors at the transcriptional level, surpassing organoids alone. Using 'omics' methods and live-cell imaging, we observed heightened responsiveness of KRAS mutant tumors to TME mechanical forces. These tumors also utilized the γ-aminobutyric acid (GABA) neurotransmitter as an energy source, increasing their invasiveness. This bioengineered model holds promise for advancing our understanding of cancer progression and improving CRC treatments.

Indexed as

invasionneurotransmittersorganoidsorgans-on-chipsperistalsis

Identifiers

PMID37745376
PMCPMC10515884
OpenAlexW4386811696

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.