Evidence map›Paper›PMID 37745402›Full record

ArticlebioRxiv : the preprint server for biology2023

U6 snRNA m6A modification is required for accurate and efficient cis- and trans-splicing of

Aykut Shen, Katarzyna Hencel, Matthew T Parker, Robyn Scott, Roberta Skukan, Aduragbemi S Adesina, Carey L Metheringham, Eric A Miska, Yunsun Nam, Wilfried Haerty and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Aykut ShenSchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
Katarzyna HencelSchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
Matthew T ParkerSchool of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
Robyn ScottDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Roberta SkukanSchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
Aduragbemi S AdesinaSchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
Carey L MetheringhamSchool of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
Eric A MiskaWellcome/CRUK Gurdon Institute, University of Cambridge, Tennis Court Rd, Cambridge, CB2 1QN, UK.
Yunsun NamDepartment of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Wilfried HaertySchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
Gordon G SimpsonSchool of Life Sciences, University of Dundee, Dow Street, Dundee, DD1 5EH, UK.
Alper AkaySchool of Biological Sciences, University of East Anglia, NR4 7TJ, Norwich.
University of East Anglia · GBThe University of Texas Southwestern Medical Center · USUniversity of Dundee · GBJames Hutton Institute · GBUniversity of Cambridge · GB

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
Mechanisms of Microprocessor Function and RegulationR01GM122960 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI NAM, YUNSUN · 2017 to 2025
$3.2M
Small molecules to target the RNA methyltransferase complex METTL3/METTL14R01CA258589 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI NAM, YUNSUN · 2022 to 2025
$1.7M
Cancer Research UK 27826Medical Research Council MR/S033769/1NCI NIH HHS R01 CA258589NIGMS NIH HHS R01 GM122960NIH HHS P40 OD010440
6 · The paper itself

Abstract

pre-mRNA splicing is a critical feature of eukaryotic gene expression. Many eukaryotes use cis-splicing to remove intronic sequences from pre-mRNAs. In addition to cis-splicing, many organisms use trans-splicing to replace the 5' ends of mRNAs with a non-coding spliced-leader RNA. Both cis- and trans-splicing rely on accurately recognising splice site sequences by spliceosomal U snRNAs and associated proteins. Spliceosomal snRNAs carry multiple RNA modifications with the potential to affect different stages of pre-mRNA splicing. Here, we show that m6A modification of U6 snRNA A43 by the RNA methyltransferase METT-10 is required for accurate and efficient cis- and trans-splicing of

Indexed as

C. elegansm6A modificationMETT-10RNA splicingSL trans-splicingSNRNP27KSNRP-27U6 snRNA

Identifiers

PMID37745402
PMCPMC10516052
OpenAlexW4386815741

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.