Evidence map›Paper›PMID 37747600›Full record

ArticlePituitary2023

Growth hormone insensitivity and adipose tissue: tissue morphology and transcriptome analyses in pigs and humans.

Jonathan A Young, Arne Hinrichs, Stephen Bell, Delaney K Geitgey, Diana Hume-Rivera, Addison Bounds, Maggie Soneson, Zvi Laron, Danielle Yaron-Shaminsky, Eckhard Wolf and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Pituitary, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 3 countries.

Jonathan A YoungEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Arne HinrichsChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, Munich, Germany.
Stephen BellEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Delaney K GeitgeyEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Diana Hume-RiveraEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Addison BoundsEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Maggie SonesonEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Zvi LaronEndocrinology and Diabetes Research Unit, Schneider Children's Medical Center, Petah Tikva, Israel.
Danielle Yaron-ShaminskyEndocrinology and Diabetes Research Unit, Schneider Children's Medical Center, Petah Tikva, Israel.
Eckhard WolfChair for Molecular Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, LMU Munich, Munich, Germany.
Edward O ListEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
John J KopchickEdison Biotechnology Institute, Ohio University, Athens, OH, USA.
Darlene E BerrymanEdison Biotechnology Institute, Ohio University, Athens, OH, USA. berrymad@ohio.edu.
Ohio University · USBiotechnology Institute · USSchneider Children's Medical Center · ILInstitute of Bioinformatics and Systems Biology · DELudwig-Maximilians-Universität München · DE

Funding

Combining GHR antagonism with life extending compounds: a search for synergiesR01AG059779 · OHIO UNIVERSITY ATHENS · 2025 to 2025
$614k
NIA NIH HHS R01 AG059779NIH HHS AG059779
6 · The paper itself

Abstract

purposeGrowth hormone receptor knockout (GHR-KO) pigs have recently been developed, which serve as a large animal model of Laron syndrome (LS). GHR-KO pigs, like individuals with LS, are obese but lack some comorbidities of obesity. The purpose of this study was to examine the histological and transcriptomic phenotype of adipose tissue (AT) in GHR-KO pigs and humans with LS.

methodsIntraabdominal (IA) and subcutaneous (SubQ) AT was collected from GHR-KO pigs and examined histologically for adipocyte size and collagen content. RNA was isolated and cDNA sequenced, and the results were analyzed to determine differentially expressed genes that were used for enrichment and pathway analysis in pig samples. For comparison, we also performed limited analyses on human AT collected from a single individual with and without LS.

resultsGHR-KO pigs have increased adipocyte size, while the LS AT had a trend towards an increase. Transcriptome analysis revealed 55 differentially expressed genes present in both depots of pig GHR-KO AT. Many significant terms in the enrichment analysis of the SubQ depot were associated with metabolism, while in the IA depot, IGF and longevity pathways were negatively enriched. In pathway analysis, multiple expected and novel pathways were significantly affected by genotype, i.e. KO vs. controls. When GH related gene expression was analyzed, SOCS3 and CISH showed species-specific changes.

conclusionAT of GHR-KO pigs has several similarities to that of humans with LS in terms of adipocyte size and gene expression profile that help describe the depot-specific adipose phenotype of both groups.

Indexed as

ObesityReceptors, SomatotropinAdipose TissueAnimalsGene Expression ProfilingGrowth HormoneHumansInsulin-Like Growth Factor ISwineGrowth HormoneInsulin-Like Growth Factor IReceptors, SomatotropinAdiposeGrowth hormoneLaron SyndromePigTranscriptome

Identifiers

PMID37747600
PMCPMC10956721
OpenAlexW4387002723

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.