Evidence map›Paper›PMID 37768452›Full record

ArticleBulletin of experimental biology and medicine2023

Development of a Method for Isolation of Mature Cardiomyocytes from Human Heart Biopsy Specimens.

S G Kovalenko, Sh R Frolova, V K Kramkova, A K Berezovskii, M A Popov, D V Shumakov, D I Zybin, E G Agafonov, V V Dontsov, K I Agladze

Abstract read
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Article in Bulletin of experimental biology and medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

S G KovalenkoResearch Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
Sh R FrolovaResearch Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
V K KramkovaLaboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
A K BerezovskiiLaboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia.
M A PopovDepartment of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
D V ShumakovDepartment of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
D I ZybinDepartment of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
E G AgafonovDepartment of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
V V DontsovDepartment of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.
K I AgladzeResearch Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia. agladze@yahoo.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To increase the yield of living cells and their survival, studies were carried out to optimize the method for isolating cardiomyocytes from biopsy specimens excised from the right atrial appendages. It was found that creatine, blebbistatin, and taurine are necessary components of the buffer solution during cardiomyocyte isolation, and that composition of the solutions is a more important factor than their oxygenation.

Indexed as

Myocytes, CardiacTaurineCell SeparationHumansTaurineelectrophysiologyimmunocytochemistry on a confocal scanning microscopeisolated human cardiomyocytesmethod of isolation

Identifiers

PMID37768452

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.