ArticleParasites & vectors2023
Assessment of the diagnostic performance of serological tests in areas where Leishmania infantum and Leishmania tarentolae occur in sympatry.
Article in Parasites & vectors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 17 citations in OpenAlex.
- First epidemiological characterization of Leishmania tarentolae in sand flies collected in southern France and Corsica Island.PLoS neglected tropical diseases · 2026Article
- Updated Canine Leishmaniosis Working Group recommendations for leishmaniosis in dogs: Q&A on clinical management.Parasites & vectors · 2026Review
- Genomic instability and mono-parental expression mitigate genomic shock in a cross-subgenusNAR molecular medicine · 2026Article
- Revisiting visceral leishmaniasis in immunocompromised patients: Ongoing gaps and advances in diagnostics, therapies, and preventive measures.Current research in parasitology & vector-borne diseases · 2026Review
- Article
- Intracellular Parasitic Infections Caused byVaccines · 2025Review
- Leishmania (Leishmania) infantum and Leishmania (Sauroleishmania) tarentolae in outdoor cats and report of infection in feline-derived peripheral blood mononuclear cells.Parasites & vectors · 2025Article
- High prevalence of vector-borne pathogens in the blood of clinically healthy dogs in Hong Kong.Parasites & vectors · 2025Article
- Cross-subgenus hybridization betweenbioRxiv : the preprint server for biology · 2025Article
- Rapid isothermal molecular tests to discriminate between Leishmania braziliensis and Leishmania infantum infections in dogs.Parasites & vectors · 2025Article
- Short Term Treatment Monitoring of Renal and Inflammatory Biomarkers with Naturally Occurring Leishmaniosis: A Cohort Study of 30 Dogs.Veterinary sciences · 2024Article
- Saurian-associated Leishmania tarentolae in dogs: Infectivity and immunogenicity evaluation in the canine model.PLoS pathogens · 2024Article
- Serum and urinary monocyte chemoattractant protein-1 as markers of inflammation and renal damage in dogs with naturally occurring leishmaniosis.Parasites & vectors · 2024Article
- Feline leishmaniosis in the Mediterranean Basin: a multicenter study.Parasites & vectors · 2024Article
- Leishmania spp. in equids and their potential vectors in endemic areas of canine leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Wildlife parasitology: sample collection and processing, diagnostic constraints, and methodological challenges in terrestrial carnivores.Parasites & vectors · 2024Review
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundVisceral leishmaniosis caused by infection with the zoonotic protozoan Leishmania infantum is a life-threatening disease affecting dogs and humans. The sympatric occurrence of L. infantum and Leishmania tarentolae in an area of southern Italy endemic for canine leishmaniosis, where dogs are also exposed to the latter species, suggests the persistence of herpetophilic L. tarentolae in a non-permissive host, therefore raising questions about the performance of serological diagnostic tests routinely employed.
methodsThe diagnostic performance of serological tests such as the immunofluorescence antibody test (IFAT), two commercial immunoenzymatic assays (i.e. NovaTec VetLine Leishmania ELISA® and rK39 ICT®) and an in-house enzyme-linked immunosorbent assay (ELISA) was evaluated in healthy dogs seropositive to L. infantum, whereas the only IFAT available was used to detect antibodies to L. tarentolae.
resultsWith the IFAT, out of a total of 104 dogs tested, 15 were seronegative for L. infantum of which three were L. tarentolae seropositive' and 89 were L. infantum seropositive. Of the latter 89 dogs, representing the highest proportion of seropositive animals (85.6%) detected by IFAT' 66 were also seropositive for L. tarentolae. Cohen's kappa (κ) agreement coefficient between the IFAT results and those of all the other tests was very low, and the IFAT results were significantly different from those of all the other serological tests as calculated by Cochran's Q-test. Analysis using the Bayesian latent class (Bayes-LCA) showed that the in-house ELISA and IFAT contributed the most towards identifying infected and non-infected dogs, respectively. The IFAT test showed low positive predictive value (59.5%), but high negative predictive value (100%).
conclusionsThese results demonstrate that the IFAT for L. infantum, although highly sensitive, may not be considered a useful diagnostic test due to its low specificity. Therefore, an accurate serological tool with high specificity is mandatory for avoiding cross-reaction in epidemiological contexts where the two species of Leishmania occur in sympatry.
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