Evidence mapPaperPMID 37808650Full record

ArticlebioRxiv : the preprint server for biology2024

A multiplexed, single-cell sequencing screen identifies compounds that increase neurogenic reprogramming of murine Muller glia.

Amy Tresenrider, Marcus Hooper, Levi Todd, Faith Kierney, Nicolai Blasdel, Cole Trapnell, Thomas A Reh

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Amy TresenriderDepartment of Genome Sciences, University of Washington, Seattle, WA 98195, USA.ORCID 0000-0002-0819-9187
Marcus HooperDepartment of Biological Structure, University of Washington, Seattle, WA 98195, USA.
Levi ToddDepartment of Biological Structure, University of Washington, Seattle, WA 98195, USA.
Faith KierneyDepartment of Biological Structure, University of Washington, Seattle, WA 98195, USA.
Nicolai BlasdelDepartment of Biological Structure, University of Washington, Seattle, WA 98195, USA.
Cole TrapnellDepartment of Genome Sciences, University of Washington, Seattle, WA 98195, USA.
Thomas A RehDepartment of Biological Structure, University of Washington, Seattle, WA 98195, USA.

Funding

Versatile, exponentially scalable methods for single cell molecular profilingR01HG010632 · UNIVERSITY OF WASHINGTON · 2025 to 2025
$655k
Stimulation of Retinal RegenerationR01EY021482 · UNIVERSITY OF WASHINGTON · 2025 to 2025
$405k
Investigation of the neuroimmune axis in retinal regeneration.K99EY033402 · NEI · UNIVERSITY OF WASHINGTON · PI Levi J. Todd · 2023 to 2023
$120k
NEI NIH HHS F32 EY032331NEI NIH HHS K99 EY033402NEI NIH HHS R01 EY021482NHGRI NIH HHS R01 HG010632
6 · The paper itself

Abstract

Retinal degeneration in mammals causes permanent loss of vision, due to an inability to regenerate naturally. Some non-mammalian vertebrates show robust regeneration, via Muller glia (MG). We have recently made significant progress in stimulating adult mouse MG to regenerate functional neurons by transgenic expression of the proneural transcription factor Ascl1. While these results showed that MG can serve as an endogenous source of neuronal replacement, the efficacy of this process is limited. With the goal of improving this in mammals, we designed a small molecule screen using sci-Plex, a method to multiplex up to thousands of single nucleus RNA-seq conditions into a single experiment. We used this technology to screen a library of 92 compounds, identified, and validated two that promote neurogenesis

Indexed as

cell signalingneuronreprogrammingretinasingle-cell sequencing

Identifiers

PMID37808650
PMCPMC10557658

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.