Evidence map›Paper›PMID 37810795›Full record

ArticlePeerJ2023

Identification and validation of hub genes involved in foam cell formation and atherosclerosis development

Da Teng, Hongping Chen, Wenjuan Jia, Qingmiao Ren, Xiaoning Ding, Lihui Zhang, Lei Gong, Hua Wang, Lin Zhong, Jun Yang

Open access · goldAbstract read
In one paragraph

Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Da Teng *Yantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Hongping Chen *Department of Cardiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
Wenjuan JiaYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Qingmiao RenThe Precision Medicine Laboratory, The First Hospital of Lanzhou University, Lanzhou, China.
Xiaoning DingYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Lihui ZhangYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Lei GongYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Hua WangYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Lin ZhongYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Jun YangYantai Yuhuangding Hospital Affiliated to Qingdao University, Yantai, China.
Qingdao University · CNYuhuangding Hospital · CNLanzhou University · CNXuzhou Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Foam cells play crucial roles in all phases of atherosclerosis. However, until now, the specific mechanisms by which these foam cells contribute to atherosclerosis remain unclear. We aimed to identify novel foam cell biomarkers and interventional targets for atherosclerosis, characterizing their potential mechanisms in the progression of atherosclerosis. Methods: Microarray data of atherosclerosis and foam cells were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expression genes (DEGs) were screened using the "LIMMA" package in R software. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and Gene Ontology (GO) annotation were both carried out. Hub genes were found in Cytoscape after a protein-protein interaction (PPI) enrichment analysis was carried out. Validation of important genes in the GSE41571 dataset, cellular assays, and tissue samples. Results: A total of 407 DEGs in atherosclerosis and 219 DEGs in foam cells were identified, and the DEGs in atherosclerosis were mainly involved in cell proliferation and differentiation. CSF1R and PLAUR were identified as common hub genes and validated in GSE41571. In addition, we also found that the expression of CSF1R and PLAUR gradually increased with the accumulation of lipids and disease progression in cell and tissue experiments. Conclusion: CSF1R and PLAUR are key hub genes of foam cells and may play an important role in the biological process of atherosclerosis. These results advance our understanding of the mechanism behind atherosclerosis and potential therapeutic targets for future development.

Indexed as

AtherosclerosisGene Expression ProfilingComputational BiologyFoam CellsHumansProtein Interaction MapsReceptor Protein-Tyrosine KinasesReceptors, Colony-Stimulating FactorReceptor Protein-Tyrosine KinasesReceptors, Colony-Stimulating FactorBioinformaticsCardiovascular disease (CVD)Foam cellsLipid metabolism

Identifiers

PMID37810795
PMCPMC10557941
OpenAlexW4387303877

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.