Evidence mapPaperPMID 37815664Full record

ArticleInternational urology and nephrology2024

Urinary stem cell-derived exocrine circRNA ATG7 regulates the SOCS1/STAT3 signaling pathway through miR-4500, inhibits M1 macrophage polarization, and alleviates the progression of diabetes nephropathy.

Yang Sun, Yanhong Zhao, Yongxin Lu, Hongmei Li, Jin Xiang, Dongmei Yang, Jinrui Wang, Xinglian Gao, Yian Wang

Open access · hybridAbstract read
In one paragraph

Article in International urology and nephrology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Exosomes applications in kidney diseases.Molecular biology reports · 2025
    Review
  7. Review
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  9. Review
  10. Review
  11. Research progress on small extracellular vesicles in diabetic nephropathy.Frontiers in cell and developmental biology · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Yang SunKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Yanhong ZhaoKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Yongxin LuKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Hongmei LiKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Jin XiangKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Dongmei YangKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Jinrui WangKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Xinglian GaoKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China.
Yian WangKidney Internal Medicine, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, 653100, Yunnan, China. wangyian@kmmu.edu.cn.ORCID http://orcid.org/0000-0001-7694-7944
Kunming Medical University · CN

Funding

Science and technology plan of Science and Technology Department of Yunnan Province - Kunming-medical joint special project 202001AY070001-258
6 · The paper itself

Abstract

objectiveThe etiopathogenesis of diabetes nephropathy (DN) has not yet been fully clarified. Finding effective treatments to prevent renal failure in DN patients has become the main focus of research in recent years. Circular RNA (circRNA) has been shown to play a momentous role in DN progression. Based on this, we aimed to investigate the potential mechanism by which urine-derived stem cell (USC)-derived exosome circRNA ATG7 (Exo-ATG7) mediates DN progression.

methodsExosomes from USCs were isolated and identified. The DN rat model was established by intraperitoneally injecting 60 mg/kg streptozotocin. The protein expression levels were measured by Western blot and immunofluorescence. HE and Masson staining were used to evaluate renal injury, and the expression of related genes was detected by RT-qPCR.

resultsCircRNA ATG7 was significantly downregulated in the DN rat model, and the extracellular vesicles of USCs improved renal function and reduced inflammation in DN rats. However, after knocking down the USCs-derived exosome circRNA ATG7, improvement and therapeutic effect on renal function in DN rats were lost. In addition, overexpression of ATG7 facilitated the switching of macrophages from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype both in vivo and in vitro. Mechanistically, upregulation of circRNA ATG7 expression can alleviate renal damage in DN rats. Importantly, the USCs-derived exosome circRNA ATG7 promotes macrophage M2 polarization by regulating the SOCS1/STAT3 signaling pathway through miR-4500. In addition, animal experiments also confirmed that after knocking down ATG7 in USC cells, the extracted exosome-treated DN rats could weaken the therapeutic effect of USC exosomes.

conclusionOur research results indicate that USC-derived exosomal circRNA ATG7 facilitates macrophage phenotype switching from M1 to M2 through the SOCS1/STAT3 signaling pathway mediated by miR-4500, thereby inhibiting DN progression.

Indexed as

Diabetes MellitusDiabetic NephropathiesExosomesMicroRNAsAnimalsAutophagy-Related Protein 7HumansMacrophagesRatsRNA, CircularSignal TransductionSTAT3 Transcription FactorStem CellsSuppressor of Cytokine Signaling 1 ProteinAtg7 protein, ratAutophagy-Related Protein 7MicroRNAsRNA, CircularSOCS1 protein, humanSTAT3 protein, humanSTAT3 Transcription FactorSuppressor of Cytokine Signaling 1 ProteinCircRNA ATG7Diabetes nephropathyMacrophagemiR-4500Urogenic stem cells

Identifiers

PMID37815664
PMCPMC10924005
OpenAlexW4387473263

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.