Ultrasound coupled RES-loaded ultrasound microbubble inhibits the proliferation of ovarian cancer cells by expression of long non-coding RNA (lncRNA) involved in apoptosis using real-time PCR.

The primary objective of this study was to evaluate the effect of low-frequency ultrasound combined with RES-loaded ultrasound microbubble contrast agents on the transcriptional and translational activities of ovarian cancer cells. After being cultures, ovarian cancer cells (OVCAR-3) and human umbilical cord endothelial cells (HUCEC) were transfected with siRNA, which was followed by RNA extraction and real-time PCR to evaluate transcriptional activity. Translational activity was determined by western blotting, which was followed by RNA interference. Proliferative and invasive activity was measured using cell proliferation, colony formation, and immunofluorescence assays. Lastly, RNA sequencing was performed. Our findings indicated that ultrasound combined with RES microbubbles inhibited cell proliferation and invasion. The expression of ING5 was enhanced, while the expression of EMT was suppressed in ovarian cancer cells. A negative correlation was observed between of the expression of ING5 and cell proliferation/migration, which were enhanced upon inhibition of ING5, suggesting dysregulation of transcriptional and translational cellular processes which could be of diagnostic and therapeutic value in ovarian cancer. Additionally, the dysregulation of lncRNAs can alter cellular homeostasis and promote ovarian cancer progression. A combination of low-frequency and RES-loaded ultrasound microbubbles was found to effectively inhibit the proliferation of OVCAR-3 ovarian cancer cells and induce apoptosis. This approach was more effective than low-frequency ultrasound combined with RES alone.