ArticleThe Plant cell2023
The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- CONSTANS-LIKE 5: a key regulator of flower opening and scent emission in Nicotiana attenuata and Petunia axillaris.The Plant cell · 2026Article
- Targeted Gene Modification ofInternational journal of molecular sciences · 2026Article
- Evolution of petal patterning: blooming floral diversity at the microscale.The New phytologist · 2025Review
- Flavor Profile of Tomatoes Across Different Cultivation Times Based on GC × GC-Q/TOFMS.Foods (Basel, Switzerland) · 2025Article
- Combined enhancement of ascorbic acid, β-carotene and zeaxanthin in gene-edited lettuce.Plant biotechnology journal · 2025Article
- Structure and release function of fragrance glands.Horticulture research · 2025Article
- Viral-Based Gene Editing System for Nutritional Improvement of Fructan Content in Lettuce.International journal of molecular sciences · 2025Article
- Metabolomic and transcriptomic analyses provide insight into the variation of floral scent and molecular regulation in different cultivars and flower development ofHorticulture research · 2025Article
- The homeotic gene PhDEF regulates production of volatiles in petunia flowers by activating EOBI and EOBII.The Plant cell · 2025Article
- Cell-type-aware regulatory landscapes governing monoterpene indole alkaloid biosynthesis in the medicinal plant Catharanthus roseus.The New phytologist · 2025Article
- Article
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The epidermal cells of petunia (Petunia × hybrida) flowers are the main site of volatile emission. However, the mechanisms underlying the release of volatiles into the environment are still being explored. Here, using cell-layer-specific transcriptomic analysis, reverse genetics by virus-induced gene silencing and clustered regularly interspaced short palindromic repeat (CRISPR), and metabolomics, we identified EPIDERMIS VOLATILE EMISSION REGULATOR (EVER)-a petal adaxial epidermis-specific MYB activator that affects the emission of volatiles. To generate ever knockout lines, we developed a viral-based CRISPR/Cas9 system for efficient gene editing in plants. These knockout lines, together with transient-suppression assays, revealed EVER's involvement in the repression of low-vapor-pressure volatiles. Internal pools and annotated scent-related genes involved in volatile production and emission were not affected by EVER. RNA-Seq analyses of petals of ever knockout lines and EVER-overexpressing flowers revealed enrichment in wax-related biosynthesis genes. Liquid chromatography/gas chromatography-MS analyses of petal epicuticular waxes revealed substantial reductions in wax loads in ever petals, particularly of monomers of fatty acids and wax esters. These results implicate EVER in the emission of volatiles by fine-tuning the composition of petal epicuticular waxes. We reveal a petunia MYB regulator that interlinks epicuticular wax composition and volatile emission, thus unraveling a regulatory layer in the scent-emission machinery in petunia flowers.
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