Evidence mapPaperPMID 37819449Full record

ArticleCellular and molecular life sciences : CMLS2023

Infiltrating macrophages amplify doxorubicin-induced cardiac damage: role of catecholamines.

Jessica Gambardella, Gaetano Santulli, Antonella Fiordelisi, Federica Andrea Cerasuolo, Xujun Wang, Nella Prevete, Eduardo Sommella, Roberta Avvisato, Antonietta Buonaiuto, Giovanna Giuseppina Altobelli and 10 more

Open access · hybridAbstract read
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
  8. Doxorubicin-Induced Cardiotoxicity: A Comprehensive Update.Journal of cardiovascular development and disease · 2025
    Review
  9. Article
  10. Review
  11. Article
  12. Article
  13. Oxidative Stress and Inflammation in Cancer.Antioxidants (Basel, Switzerland) · 2024
    Article
  14. Review
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors at 4 institutions in 2 countries.

Jessica GambardellaDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Gaetano SantulliDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Antonella FiordelisiDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Federica Andrea CerasuoloDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Xujun WangDepartment of Medicine (Cardiology) and Department of Molecular Pharmacology, Wilf Family Cardiovascular Research Institute, Albert Einstein College of Medicine, Montefiore University Hospital, New York, USA.
Nella PreveteDepartment of Translational Medical Sciences, Federico II University, Naples, Italy.
Eduardo SommellaDepartment of Pharmacy, University of Salerno, Fisciano (Salerno), Italy.
Roberta AvvisatoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Antonietta BuonaiutoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Giovanna Giuseppina AltobelliDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Laura RinaldiDepartment of Molecular Medicine and Medical Biotechnology, Federico II University, Naples, Italy.
Francesco ChiusoDepartment of Molecular Medicine and Medical Biotechnology, Federico II University, Naples, Italy.
Antonio FelicielloDepartment of Molecular Medicine and Medical Biotechnology, Federico II University, Naples, Italy.
Fabrizio Dal PiazDepartment of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno (Salerno), Baronissi, Italy.
Pietro CampigliaDepartment of Pharmacy, University of Salerno, Fisciano (Salerno), Italy.
Michele CiccarelliDepartment of Medicine, Surgery and Dentistry "Scuola Medica Salernitana", University of Salerno (Salerno), Baronissi, Italy.
Carmine MoriscoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Junichi SadoshimaDepartment of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers University, Newark, New Jersey, USA.
Guido IaccarinoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy.
Daniela SorrientoDepartment of Advanced Biomedical Sciences, Federico II University, Naples, Italy. daniela.sorriento@unina.it.ORCID http://orcid.org/0000-0002-1599-4509
Federico II University Hospital · ITUniversity of Salerno · ITAlbert Einstein College of Medicine · USRutgers, The State University of New Jersey · US

Funding

AIRC IG 2018-ID22062MIUR PRIN 2017HTKLRFRegione Campania PON03PE_00060_8
6 · The paper itself

Abstract

backgroundThe functional contribution of non-myocyte cardiac cells, such as inflammatory cells, in the setup of heart failure in response to doxorubicin (Dox) is recently becoming of growing interest.

objectivesThe study aims to evaluate the role of macrophages in cardiac damage elicited by Dox treatment.

methodsC57BL/6 mice were treated with one intraperitoneal injection of Dox (20 mg/kg) and followed up for 5 days by cardiac ultrasounds (CUS), histological, and flow cytometry evaluations. We also tested the impact of Dox in macrophage-depleted mice. Rat cardiomyoblasts were directly treated with Dox (D-Dox) or with a conditioned medium from cultured murine macrophages treated with Dox (M-Dox).

resultsIn response to Dox, macrophage infiltration preceded cardiac damage. Macrophage depletion prevents Dox-induced damage, suggesting a key role of these cells in promoting cardiotoxicity. To evaluate the crosstalk between macrophages and cardiac cells in response to DOX, we compared the effects of D-Dox and M-Dox in vitro. Cell vitality was lower in cardiomyoblasts and apoptosis was higher in response to M-Dox compared with D-Dox. These events were linked to p53-induced mitochondria morphology, function, and autophagy alterations. We identify a mechanistic role of catecholamines released by Dox-activated macrophages that lead to mitochondrial apoptosis of cardiac cells through β-AR stimulation.

conclusionsOur data indicate that crosstalk between macrophages and cardiac cells participates in cardiac damage in response to Dox.

Indexed as

CatecholaminesDoxorubicinAnimalsApoptosisMacrophagesMiceMice, Inbred C57BLMyocytes, CardiacOxidative StressRatsCatecholaminesDoxorubicinCatecholaminesDoxorubicinMacrophagesMitochondriaMitophagyp53β-AR

Identifiers

PMID37819449
PMCPMC10567889
OpenAlexW4387529360

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.