Evidence map›Paper›PMID 37820720›Full record

ArticleAdvanced healthcare materials2024

NanoIEA: A Nanopatterned Interdigitated Electrode Array-Based Impedance Assay for Real-Time Measurement of Aligned Endothelial Cell Barrier Functions.

Jong Seob Choi, Hyun Myung Doo, Byunggik Kim, Su Han Lee, Sang-Keun Sung, Gwangjun Go, Allister Suarez, Yeseul Kim, Byung Mook Weon, Byung-Ok Choi and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced healthcare materials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 6 institutions in 2 countries.

Jong Seob ChoiDepartment of Biomedical Engineering, Center for Microphysiological Systems, Johns Hopkins University, Baltimore, MD, 21205, USA.
Hyun Myung DooDepartment of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, South Korea.ORCID 0000-0001-7419-5005
Byunggik KimDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 21218, USA.
Su Han LeeDigital Health Care Research Center, Gumi Electronics and Information Technology Research Institute (GERI), Gumi, Gyeongbuk, 39253, South Korea.ORCID 0009-0003-2396-809X
Sang-Keun SungDigital Health Care Research Center, Gumi Electronics and Information Technology Research Institute (GERI), Gumi, Gyeongbuk, 39253, South Korea.
Gwangjun GoDepartment of Biomedical Engineering, Center for Microphysiological Systems, Johns Hopkins University, Baltimore, MD, 21205, USA.
Allister SuarezDepartment of Biomedical Engineering, Center for Microphysiological Systems, Johns Hopkins University, Baltimore, MD, 21205, USA.
Yeseul KimSKKU Advanced Institute of Nanotechnology (SAINT), School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, 16419, South Korea.
Byung Mook WeonSKKU Advanced Institute of Nanotechnology (SAINT), School of Advanced Materials Science and Engineering, Sungkyunkwan University, Suwon, 16419, South Korea.
Byung-Ok ChoiDepartment of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul, 06351, South Korea.
Hyung Jin KimSchool of Electrical and Electronic Engineering, Ulsan College, Ulsan, 44610, South Korea.
Deok-Ho KimDepartment of Biomedical Engineering, Center for Microphysiological Systems, Johns Hopkins University, Baltimore, MD, 21205, USA.ORCID 0000-0002-6989-6074
Johns Hopkins University · USGumi Electronics & Information Technology Research Institute (South Korea) · KRSungkyunkwan University · KRKorea University Medical Center · KRSamsung Medical Center · KRUlsan College · KR

Funding

A NANOPATTERNED MEA-BASED FUNCTIONAL PHENOTYPING ASSAY FOR DRUG-INDUCED CARDIOTOXICITY SCREENINGR01HL135143 · NHLBI · UNIVERSITY OF WASHINGTON · PI KIM, DEOK-HO · 2017 to 2021
$1.9M
Tissue-Engineered Models of Lymphatic Drainage in Breast CancerR01CA279560 · NCI · CORNELL UNIVERSITY · PI Esak Lee · 2023 to 2026
$1.6M
High-throughput nanoIEA-based Assay for Screening Immune Cell-Vascular InteractionsR21AI168886 · NIAID · CORNELL UNIVERSITY · PI KIM, DEOK-HO, LEE, ESAK · 2023 to 2024
$450k
NCI NIH HHS R01 CA279560NHLBI NIH HHS R01 HL135143NIAID NIH HHS R21 AI168886NIH HHS R01HL135143NIH HHS R21AI168886
6 · The paper itself

Abstract

A nanopatterned interdigitated electrode array (nanoIEA)-based impedance assay is developed for quantitative real-time measurement of aligned endothelial cell (EC) barrier functions in vitro. A bioinspired poly(3,4-dihydroxy-L-phenylalanine) (poly (l-DOPA)) coating is applied to improve the human brain EC adhesion onto the Nafion nanopatterned surfaces. It is found that a poly (l-DOPA)-coated Nafion grooved nanopattern makes the human brain ECs orient along the nanopattern direction. Aligned human brain ECs on Nafion nanopatterns exhibit increased expression of genes encoding tight and adherens junction proteins. Aligned human brain ECs also have enhanced impedance and resistance versus unaligned ones. Treatment with a glycogen synthase kinase-3 inhibitor (GSK3i) further increases impedance and resistance, suggesting synergistic effects occur on the cell-cell tightness of in vitro human brain ECs via a combination of anisotropic matrix nanotopography and GSK3i treatment. It is found that this enhanced cell-cell tightness of the combined approach is accompanied by increased expression of claudin protein. These data demonstrate that the proposed nanoIEA assay integrated with poly (l-DOPA)-coated Nafion nanopatterns and interdigitated electrode arrays can make not only biomimetic aligned ECs, but also enable real-time measurement of the enhanced barrier functions of aligned ECs via tighter cell-cell junctions.

Indexed as

Endothelial CellsFluorocarbon PolymersLevodopaElectric ImpedanceEndotheliumHumansFluorocarbon PolymersLevodopaperfluorosulfonic acidendothelial barrier functionimpedance assaysinterdigitated electrodesnanopatterned Nafionpoly (L-DOPA)

Identifiers

PMID37820720
PMCPMC10841753
OpenAlexW4387523683

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.