Evidence mapPaperPMID 37822188Full record

ArticleJournal of biophotonics2024

Demonstrating drug treatment efficacies by monitoring superoxide dynamics in human lung cancer cells with time-lapse fluorescence microscopy.

Shalaka Konjalwar, Busenur Ceyhan, Oscar Rivera, Parisa Nategh, Mehrnoosh Neghabi, Mirjana Pavlovic, Shailaja Allani, Mahsa Ranji

Open access · greenAbstract read
In one paragraph

Article in Journal of biophotonics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. In-vehicle Sensing and Data Analysis for Older Drivers with Mild Cognitive Impairment.2023 IEEE 20th International Conference on Smart Communities: Improving Quality of Life using AI, Robotics and IoT (HONET) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Shalaka KonjalwarDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0009-0009-2868-1833
Busenur CeyhanDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0009-0001-8549-2846
Oscar RiveraCenter for Molecular Biology and Biotechnology, Florida Atlantic University, Boca Raton, Florida, USA.
Parisa NateghDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0009-0009-2974-7742
Mehrnoosh NeghabiDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0000-0003-1256-4770
Mirjana PavlovicDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.
Shailaja AllaniCenter for Molecular Biology and Biotechnology, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0000-0002-1900-2933
Mahsa RanjiDepartment of Electrical Engineering and Computer Science, Florida Atlantic University, Boca Raton, Florida, USA.ORCID 0000-0002-4185-5676
Florida Atlantic University · US

Funding

NEI NIH HHS R15 EY031533NIH HHS EY031533
6 · The paper itself

Abstract

Metformin hydrochloride, an antihyperglycemic agent, and sulindac, a nonsteroidal anti-inflammatory drug, are FDA-approved drugs known to exert anticancer effects. Previous studies demonstrated sulindac and metformin's anticancer properties through mitochondrial dysfunction and inhibition of mitochondrial electron transport chain complex I and key signaling pathways. In this study, various drugs were administered to A549 lung cancer cells, and results revealed that a combination of sulindac and metformin enhanced cell death compared to the administration of the drugs separately. To measure superoxide production over time, we employed a time-lapse fluorescence imaging technique using mitochondrial-targeted hydroethidine. Fluorescence microscopy data showed the most significant increases in superoxide production in the combination treatment of metformin and sulindac. Results showed significant differences between the combined drug treatment and control groups and between the positive control and control groups. This approach can be utilized to quantify the anticancer efficacy of drugs, creating possibilities for additional therapeutic options.

Indexed as

Lung NeoplasmsMetforminCell Line, TumorHumansMicroscopy, FluorescencePharmaceutical PreparationsSulindacSuperoxidesTime-Lapse ImagingMetforminPharmaceutical PreparationsSulindacSuperoxidesfluorescence microscopymetforminMitoSOX Redoxidative stresssulindacsuperoxidetime-lapse imaging

Identifiers

PMID37822188
PMCPMC12013861
OpenAlexW4387562813

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.