Evidence map›Paper›PMID 37828427›Full record

ArticleCellular & molecular biology letters2023

Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice.

Huimeng Qi, Zhaoguo Zheng, Qiang Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular & molecular biology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Huimeng Qi *Department of General Practice, Fuyang Hospital of Anhui Medical University, Fuyang, 236000, Anhui, People's Republic of China.
Zhaoguo Zheng *Department of Nephrology, Guangdong Second Provincial General Hospital, Haizhu District, No. 466, Xingang Zhong, Guangzhou, 510317, Guangdong, People's Republic of China.
Qiang LiuDepartment of Nephrology, Guangdong Second Provincial General Hospital, Haizhu District, No. 466, Xingang Zhong, Guangzhou, 510317, Guangdong, People's Republic of China. liuqiang@ahmu.edu.cn.
Guangdong Provincial People's Hospital · CNFuyang Second People's Hospital · CN

Funding

Anhui Medical University Basic and Clinical Cooperative Research Improvement Program Project 2022xkjT039Anhui Medical University Clinical Medicine Discipline Co construction Project, Anhui Medical University Peak Discipline Construction Project, Anhui Medical University Basic and Clinical Cooperative Research Improvement Program Project 2022xkjT038General projects of Anhui Provincial Health Commission AHWJ2022b059Scientific Research Fund of Translational Medicine Research Institute of Anhui Province, Clinical Science Foundation Project of Anhui Medical University 2022xkj216
6 · The paper itself

Abstract

backgroundLupus nephritis (LN) is associated with significant mortality and morbidity, while effective therapeutics and biomarkers are limited since the pathogenesis is complex. This study investigated the roles of the CEBPB/BZW1/eIF2α axis in metabolic reprogramming and endoplasmic reticulum stress in LN.

methodThe differentially expressed genes in LN were screened using bioinformatics tools. The expression of CEBPB in the renal tissue of patients with LN and its correlation with the levels of creatinine and urinary protein were analyzed. We used adenoviral vectors to construct LN mice with knockdown CEBPB using MRL/lpr lupus-prone mice and analyzed the physiological and autoimmune indices in mice. Chromatin immunoprecipitation quantitative polymerase chain reaction (ChIP-qPCR) and dual-luciferase reporter assays were conducted to explore the regulation of BZW1 by CEBPB, followed by glycolytic flux analysis, glucose uptake, and enzyme-linked immunosorbent assay (ELISA). Finally, the role of eIF2α phosphorylation by BZW1 in bone marrow-derived macrophages (BMDM) was explored using eIF2α phosphorylation and endoplasmic reticulum stress inhibitors.

resultsCEBPB was significantly increased in renal tissues of patients with LN and positively correlated with creatinine and urine protein levels in patients. Downregulation of CEBPB alleviated the autoimmune response and the development of nephritis in LN mice. Transcriptional activation of BZW1 by CEBPB-mediated glucose metabolic reprogramming in macrophages, and upregulation of BZW1 reversed the mitigating effect of CEBPB knockdown on LN. Regulation of eIF2α phosphorylation levels by BZW1 promoted endoplasmic reticulum stress-amplified inflammatory responses in BMDM.

conclusionTranscriptional activation of BZW1 by CEBPB promoted phosphorylation of eIF2α to promote macrophage glycolysis and endoplasmic reticulum stress in the development of LN.

Indexed as

Endoplasmic Reticulum StressLupus NephritisAnimalsCCAAT-Enhancer-Binding Protein-betaCell Cycle ProteinsCreatinineDNA-Binding ProteinsEukaryotic Initiation Factor-2HumansMacrophagesMiceMice, Inbred MRL lprPhosphorylationBZW1 protein, humanBZW1 protein, mouseCCAAT-Enhancer-Binding Protein-betaCEBPB protein, humanCell Cycle ProteinsCreatinineDNA-Binding ProteinsEIF2S1 protein, humanEukaryotic Initiation Factor-2BZW1CEBPBEndoplasmic reticulum stressGlycolysisLupus nephritis

Identifiers

PMID37828427
PMCPMC10571419
OpenAlexW4387581662

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.