Evidence map›Paper›PMID 37834997›Full record

ArticleJournal of clinical medicine2023

Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study.

Carlos Rocha-de-Lossada, Cosimo Mazzotta, Federico Gabrielli, Filomena Tiziana Papa, Carmen Gómez-Huertas, Celia García-López, Facundo Urbinati, Rahul Rachwani-Anil, María García-Lorente, José-María Sánchez-González and 3 more

Abstract read
In one paragraph

Article in Journal of clinical medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Carlos Rocha-de-LossadaEyemetagenomics Ltd., 71-75, Shelton Street, Covent Garden, London WC2H 9JQ, UK.ORCID 0000-0001-7464-2493
Cosimo MazzottaSiena Crosslinking Center, 53100 Siena, Italy.
Federico GabrielliBiolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy.
Filomena Tiziana PapaBiolab SRL, Laboratorio di Genetica e Genomica Molecolare, Largo degli Aranci, 9, 63100 Ascoli Piceno, Italy.ORCID 0000-0001-7821-8198
Carmen Gómez-HuertasDepartment of Ophthalmology, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain.
Celia García-LópezDepartment of Ophthalmology, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain.
Facundo UrbinatiOphthalmology Department, Hospital Regional Universitario Málaga, 29010 Malaga, Spain.ORCID 0000-0002-1288-3715
Rahul Rachwani-AnilDepartment of Ophthalmology, Hospital de Antequera, 29200 Malaga, Spain.ORCID 0000-0001-5901-2469
María García-LorenteOphthalmology Department, Hospital Regional Universitario Málaga, 29010 Malaga, Spain.
José-María Sánchez-GonzálezDepartment of Physics of Condensed Matter, Optics Area, University of Seville, 41012 Seville, Spain.ORCID 0000-0003-0450-7717
Miguel RechichiCentro Polispecialistico Mediterraneo, 88050 Sellia Marina, Italy.ORCID 0000-0002-1016-2535
Giovanni RubegniPostgraduate Ophthalmology School, University of Siena, 53100 Siena, Italy.
Davide BorroniEyemetagenomics Ltd., 71-75, Shelton Street, Covent Garden, London WC2H 9JQ, UK.ORCID 0000-0001-6952-5647

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development.

Indexed as

keratoconusmetagenomicsmicrobiotavalidation-study

Identifiers

PMID37834997
PMCPMC10573816

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.