Evidence map›Paper›PMID 37848864›Full record

ArticleBMC genomics2023

Identification and characterization of differentially expressed circular RNAs in extraocular muscle of oculomotor nerve palsy.

Mingsu Shi, Yanxi Fang, Yu Liang, Yuxiang Hu, Jiaqiu Huang, Weiyi Xia, Hewei Bian, Qiao Zhuo, Lianqun Wu, Chen Zhao

Abstract read
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In one paragraph

Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Mingsu Shi *Eye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Yanxi Fang *Eye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Yu LiangEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Yuxiang HuEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Jiaqiu HuangEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Weiyi XiaEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Hewei BianEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Qiao ZhuoEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Lianqun WuEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China. lianqun.wu@fdeent.org.
Chen ZhaoEye Institute, Department of Ophthalmology, Eye & ENT Hospital, Fudan University, 83 Fenyang Road, Shanghai, 200031, China. dr_zhaochen@163.com.

Funding

National Natural Science Foundation of China 82020108006, 81730025, 81670864 and 81525006 to C. ZNational Natural Science Foundation of China 82271126, 81600765 to LQ. WNatural Science Foundation of Shanghai 20ZR1409800 to LQ. W
6 · The paper itself

Abstract

backgroundOculomotor nerve palsy (ONP) is a neuroparalytic disorder resulting in dysfunction of innervating extraocular muscles (EOMs), of which the pathological characteristics remain underexplored.

methodsIn this study, medial rectus muscle tissue samples from four ONP patients and four constant exotropia (CXT) patients were collected for RNA sequencing. Differentially expressed circular RNAs (circRNAs) were identified and included in functional enrichment analysis, followed by interaction analysis with microRNAs and mRNAs as well as RNA binding proteins. Furthermore, RT-qPCR was used to validate the expression level of the differentially expressed circRNAs.

resultsA total of 84 differentially expressed circRNAs were identified from 10,504 predicted circRNAs. Functional enrichment analysis indicated that the differentially expressed circRNAs significantly correlated with skeletal muscle contraction. In addition, interaction analyses showed that up-regulated circRNA_03628 was significantly interacted with RNA binding protein AGO2 and EIF4A3 as well as microRNA hsa-miR-188-5p and hsa-miR-4529-5p. The up-regulation of circRNA_03628 was validated by RT-qPCR, followed by further elaboration of the expression, location and clinical significance of circRNA_03628 in EOMs of ONP.

conclusionsOur study may shed light on the role of differentially expressed circRNAs, especially circRNA_03628, in the pathological changes of EOMs in ONP.

Indexed as

MicroRNAsRNA, CircularDEAD-box RNA HelicasesEukaryotic Initiation Factor-4AHumansOculomotor MusclesRNA, MessengerSequence Analysis, RNAUp-RegulationDEAD-box RNA HelicasesEIF4A3 protein, humanEukaryotic Initiation Factor-4AMicroRNAsMIRN188 microRNA, humanRNA, CircularRNA, MessengerCircular RNAConstant exotropiaExtraocular muscleOculomotor nerve palsy

Identifiers

PMID37848864
PMCPMC10583365

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.